nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2008-12-1-58-63nefr-1015Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. EXPERIMENTAL INVESTIGATIONО РОЛИ ПРОЦЕСОВ СВОБОДНО-РАДИКАЛЬНОГО ОКИСЛЕНИЯ В РАЗВИТИИ ЭКСПЕРИМЕНТАЛЬНОГО НЕФРОЛИТИАЗАON THE ROLE OF PROCESSES OF FREE RADICAL OXIDATION IN THE DEVELOPMENT OF EXPERIMENTAL NEPHROLITHIASISЗверевЯ. Ф.ZverevYa. F.

кафедры фармакологии и гистологии

БрюхановВ. М.BryukhanovV. M.

кафедры фармакологии и гистологии

ТалалаеваО. С.TalalaevaO. S.

кафедры фармакологии и гистологии

ЛампатовВ. В.LampatovV. V.

кафедры фармакологии и гистологии

ЖариковА. Ю.ZharikovA. Yu.

кафедры фармакологии и гистологии

ТалалаевС. В.TalalaevS. V.

кафедры фармакологии и гистологии

БулгаковаЯ. С.BulgakovaYa. S.

кафедры фармакологии и гистологии

Алтайский государственный медицинский университетRussian Federation2008100120081215863Copyright © Зверев Я.Ф., Брюханов В.М., Талалаева О.С., Лампатов В.В., Жариков А.Ю., Талалаев С.В., Булгакова Я.С., 20082008Зверев Я.Ф., Брюханов В.М., Талалаева О.С., Лампатов В.В., Жариков А.Ю., Талалаев С.В., Булгакова Я.С.Zverev Y.F., Bryukhanov V.M., Talalaeva O.S., Lampatov V.V., Zharikov A.Y., Talalaev S.V., Bulgakova Y.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/1015

ЦЕЛЬЮ ИССЛЕДОВАНИЯ явилось изучение процесса свободно-радикального окисления в почках и крови крыс с экспериментальной мочекаменной болезнью. МАТЕРИАЛ И МЕТОДЫ. Эксперименты проведены на самцах крыс Wistar, получавших в виде питья на протяжении 21 дня 1%-й раствор этиленгликоля. Гистохимически в почечных срезах определяли наличие кристаллов оксалата кальция. Биохимическими методами оценивали оксидантный (тиобарбитуратреактивные продукты, общая оксидантная активность) и антиоксидантный (каталаза, супероксиддисмутаза, глутатионпероксидаза, общая антиоксидантная активность) статусы почек и крови. РЕЗУЛЬТАТЫ. В ходе экспериментов в срезах почек всех крыс, потреблявших этиленгликоль, зафиксировали значительное количество Са-позитивных кристаллов, локализованных преимущественно на поверхности почечного сосочка. Развитие нефролитиаза сопровождалось мощной активацией свободно-радикального окисления как в почках, так и в крови животных. Одновременно выявлялось ослабление антиоксидантной защиты, обусловленное в основном угнетением активности глутатионпероксидазы. ЗАКЛЮЧЕНИЕ. Таким образом, в условиях экспериментального оксалатного нефролитиаза у крыс происходит активация свободно-радикального окисления в почках и крови животных. Этот процесс сопровождается ослаблением активности глутатионпероксидазы, одного из основных антиоксидантных ферментов.

THE AIM of the investigation was to study the process of free-radical oxidation in the kidneys and blood of rats with experimental urolithiasis. MATERIAL AND METHODS. Experiments were carried out in male Wistar rats given 1% solution of ethylene glycol in drinking water during 21 days. The presence of calcium oxalate crystals in kidney slices was determined histochemically Biochemical methods were used to assess the oxidant (thiobarbiturate reactive products, total oxidant activity) and antioxidant (catalase, superoxiddismutase, glutathione peroxidase, total antioxidant activity) status of the kidneys and blood. RESULTS. Considerable number of Ca-positive crystals localized mainly on the surface of the renal papilla was determined in kidney slices of all rats receiving ethylene glycol. Progression of nephrolithiasis was accompanied by intense activation of free-radical oxidation both in the kidneys and in blood. Abatement of antioxidant defense was simultaneously observed due to the inhibition of activity of glutathione peroxidase. CONCLUSION. Thus, activation of free-radical oxidation in the kidneys and  blood of animals takes place under conditions of experimental oxalate nephrolithiasis in rats. This process is followed by weakened activity of glutathione peroxidase as one of the main antioxidant enzymes.

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The authors declare that there are no conflicts of interest present.