nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2009-13-2-65-74nefr-1165Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. CLINICAL INVESTIGATIONSПРАКТИЧЕСКОЕ ЗНАЧЕНИЕ АНАЛИЗА ВЕЛИЧИНЫ ОСМОЛЯЛЬНОСТИ СЛЮНЫ ЧЕЛОВЕКА В НОРМЕ И ПРИ ПАТОЛОГИИTHE PRACTICAL SIGNIFICANSE OF DETERMINATION OF THE OSMOLALITY OF THE SALIVA IN HEALTHY AND ILL PATIENTSЗапорожанВ. Н.ZaporzhanV. P.

Научно-исследовательский институт молекулярно-генетической и клинической  медицины

ДоломатовС. И.DolomatovS. I.

Научно-исследовательский институт молекулярно-генетической и клинической  медицины

65026, Одесса, Украина, Валиховский переулок, 2, Одесский государственный медицинский университет

dolomatov@rambler.ruЖуковВ. А.ZhukovV. A.

Научно-исследовательский институт молекулярно-генетической и клинической  медицины

АтмажовИ. Д.AtmazhovI. Z.

кафедра черепно-лицевой хирургии

Одесский государственный медицинский университетUkraine2009100220091326574Copyright © Запорожан В.Н., Доломатов С.И., Жуков В.А., Атмажов И.Д., 20092009Запорожан В.Н., Доломатов С.И., Жуков В.А., Атмажов И.Д.Zaporzhan V.P., Dolomatov S.I., Zhukov V.A., Atmazhov I.Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/1165

ЦЕЛЬ РАБОТЫ исследование динамики показателей осмоляльности слюны женщин с патологическим течением беременности и при парадонтите на фоне беременности. ПАЦИЕНТЫ И МЕТОДЫ. Обследованы 4 группы женщин: контрольная группа небеременных женщин репродуктивного возраста, беременные с физиологически протекающей беременностью (24-28 недель) без признаков заболеваний пародонта (10 человек), беременные с генерализованным пародонтитом I-II степени в период ремиссии (10 человек), женщины с патологическим течением беременности без признаков воспаления пародонта (10 человек). В каждой из групп собирали по 2 пробы слюны, первый сбор слюны проводили утром, натощак. Затем назначали полоскание полости рта 0,9% раствором хлорида натрия и через 30 мин. – вторую порцию слюны. В образцах слюны определяли величину осмоляльности, концентрацию нитритов, общего кальция и неорганических фосфатов, а также содержание молекул средней массы (λ=254нм и 280нм). Методом иммуноферментного анализа в образцах слюны контрольных групп небеременных и беременных, а также женщин с признаками патологического течения беременности определяли содержание эстриола, тестостерона и дегидроэпиандростерон-сульфата. РЕЗУЛЬТАТЫ. Установлено, что слюна является гипоосмотической жидкостью, а полоскание полости рта 0,9% раствором хлорида натрия, в группе практически здоровых небеременных женщин, приводит к снижению осмоляльности слюны. Показано, что физиологическое течение беременности сопровождается снижением содержания в слюне осмотически активных веществ, нитритов и общего кальция, а полоскание полости рта 0,9% раствором хлорида натрия не вызывает уменьшения концентрации осмотически активных веществ в слюне. При патологическом течении беременности не происходит характерного для физиологически протекающей беременности понижения величины содержания в слюне осмотически активных веществ и нитритов. В слюне беременных с парадонтитом регистрируется максимально высокое содержание нитритов и молекул средней массы, а также устойчивое повышение осмоляльности слюны в сравнении со здоровыми беременными. Установлено, что течение парадонтита на фоне беременности характеризуется более высоким уровнем в слюне ДГЭА-сульфата и снижением эстриола, в сравнении с контрольной группой беременных. ЗАКЛЮЧЕНИЕ. Исследование осмотического состава слюны может дать ценную информацию о состоянии полости рта при физиологическом и патологическом течении беременности.

THE AIM is the study of the data dynamics of osmolality of saliva of women with pathologic pregnancy and with paradontitis during pregnancy. PATIENTS AND METHODS. 4 groups of women were investigated: the control group of non pregnant women of the reproductive age, pregnant women with physiologic pregnancy (24-28 weeks) without the signs of paradontitis (10 people, pregnant women with generalized paradontitis I-II stages in the time of remission (10 people), women with pathologic pregnancy without the signs of inflammatory paradont (10 patients). From each group 2 saliva tests were collected, the first test was taken in the morning on the empty stomach. Then mouth wash was prescribed with the 0,9 % saline solution and after 30 minutes the second test was taken. In the saliva tests the value of osmolality, nitrite concentration, total calcium and nonorganic phosphates, and also the concentration of medium mass molecules were determined (λ = 254 nm and 280 nm). By means of immunoferment analysis the amount of estradiol, testosteron, and dehydroepiandrosteron-sulfate in saliva tests of the control group of nonpregnant and pregnant women, and also in women with the sings of pathologic pregnancy were evaluated. RESULTS. Was determined that saliva is hyperosmic fluid, and mouth wash with 0,9% saline solution in the group of health nonpregnant women leads to the decrease in osmolality of saliva. Also was noted that physiologic flow of pregnancy leads to the decrease in saliva of somatically active substances, nitrates and total calcium, and the mouth rinse with the 0,9% saline solution does not lead to the decrease in the concentration of osmotically active substances in saliva. In pathologic pregnancy flow the characteristic for the physiological pregnancy decrease of the value of the concentration of osmotically active substances and nitrates does not take place. In the saliva of pregnant women with paradontitis the concentration of nitrates and medium weight molecules are at their highest, and also a stable increase of osmolality of saliva in comparison with health women take place. It was noted that the paradontitis flow during the pregnancy is characterized by a higher level in saliva of DHEA-sulfate and the decrease of estradiol, in comparison with control group of pregnant women. CONCLUSION. The investigation of osmotic consistence of saliva can give a valuable information about the state of the mouth cavity state during physiologic and pathologic pregnancy flow.

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The authors declare that there are no conflicts of interest present.