nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2009-13-4-51-58nefr-1190Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. CLINICAL INVESTIGATIONSИЗУЧЕНИЕ ЛОКАЛИЗАЦИИ РЕЦЕПТОРОВ ВАЗОПРЕССИНА В ПОЧКЕ ДЕТЕЙ ПРИ ГЛОМЕРУЛОНЕФРИТЕSTUDY OF LOCALIZATION OF VASOPRESSIN RECEPTORS IN KIDNEY OF CHILDREN WITH GLOMERULONEPHRITISСеливёрстоваЕ. В.SeliverstovaE. V.

лаборатория физиологии почки и водно-солевого обмена

194223, Санкт-Петербург, пр. Тореза, 44; тел. 552-30-86.

natochin@iephb.ruСоловьёвА. А.SolovyovA. A.

кафедра госпитальной педиатрии

НасыровР. А.NasyrovR. A.

кафедра патологической анатомии с курсом судебной медицины

НаточинЮ. В.NatochinYu. V.

лаборатория физиологии почки и водно-солевого обмена

Институт эволюционной физиологии и биохи­мии им. И.М.Сеченова РАНRussian FederationСанкт-Петербургская государ­ственная медицинская педиатрическая академияRussian Federation2009100420091345158Copyright © Селивёрстова Е.В., Соловьёв А.А., Насыров Р.А., Наточин Ю.В., 20092009Селивёрстова Е.В., Соловьёв А.А., Насыров Р.А., Наточин Ю.В.Seliverstova E.V., Solovyov A.A., Nasyrov R.A., Natochin Y.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/1190

ЦЕЛЬ ИССЛЕДОВАНИЯ. Изучение локализации рецепторов вазопрессина в структурах почки человека. ПАЦИЕНТЫ И МЕТОДЫ. Исследованы биоптаты почек 12 детей с различными морфологическими формами гломерулонефрита. Локализация V1a- и V2-рецепторов изучена методами световой и электронной микроскопии, иммуногистохимии и иммуноэлектронной микроскопии. РЕЗУЛЬТАТЫ. В клубочках и эпителии проксимальных канальцев нефрона были выявлены изменения ультраструктуры, клетки дистального сегмента нефрона не имели выраженных изменений. Использование специфических антител позволило установить, что V2-рецепторы локализованы в клетках собирательных трубок и дистального сегмента нефрона, V1a-рецепторы – в клетках мезангия клубочка, стенок сосудов, дистального сегмента нефрона и интерстиция. ЗАКЛЮЧЕНИЕ. Впервые представлены данные о локализации V1a- и V2-рецепторов в почке детей. Выявленное распределение V-рецепторов в структурах почки хорошо сопоставимо с результатами изучения функционального ответа почек на вазопрессин.

THE AIM. Study of localization of vasopressin receptors in the structures of human kidney. PATIENTS AND METHODS. Renal tissue studied 12 children with different morphological forms of glomerulonephritis. Localization of V1a- and V2-receptor studied by light and electron microscopy, immunohistochemistry and immunoelectron microscopy. RESULTS. In the glomeruli and the epithelium of the proximal tubules of the nephron were identified changes in the ultrastructure of cells. The cells of distal segment of the nephron were not expressed by the changes. Using specific antibodies revealed that the V2-receptors localized in the cells of the collecting duct and the distal segment of the nephron, V1a-receptors – in the mesangial cells, the walls of blood vessels, the distal segment of the nephron and interstitium. CONCLUSION. For the first time presents data on the localization of V1a- and V2-receptors in the kidneys of children. The observed distribution of V-receptors in the structures of the kidney are well comparable with the results of the study of the functional response of the kidneys to vasopressin.

вазопрессинV1a-рецепторV2-рецепторкора почкинефрониммуногистохимияиммуноэлектронная микроскопияvasopressinV1a-receptorV2-receptorkidney cortexnephronimmunohistochemistryimmunoelectron microscopyReferencesAmmar A, Roseau S, Butlen D. Pharmacological characterization of V1a vasopressin receptors in the rat cortical collecting duct. Am J Physiol 1992; 262 (4 Pt 2): F546–F553Ammar A, Roseau S, Butlen D. Pharmacological characterization of V1a vasopressin receptors in the rat cortical collecting duct. Am J Physiol 1992; 262 (4 Pt 2): F546–F553Nonoguchi H, Owada A, Kobayashi N et al. Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts. J Clin Invest 1995; 96 (4): 1768-1778Nonoguchi H, Owada A, Kobayashi N et al. Immunohistochemical localization of V2 vasopressin receptor along the nephron and functional role of luminal V2 receptor in terminal inner medullary collecting ducts. J Clin Invest 1995; 96 (4): 1768-1778Zhuo JL. Renomedullary interstitial cells: a target for endocrine and paracrine actions of vasoactive peptides in the renal medulla. Clin Exp Pharmacol Physiol 2000; 27 (7): 465-473Zhuo JL. Renomedullary interstitial cells: a target for endocrine and paracrine actions of vasoactive peptides in the renal medulla. Clin Exp Pharmacol Physiol 2000; 27 (7): 465-473Tashima Y, Kohda Y, Nonoguchi H et al. Intranephron localization and regulation of the V1a vasopressin receptor during chronic metabolic acidosis and dehydration in rats. Pflugers Arch 2001; 442 (5): 652-661Tashima Y, Kohda Y, Nonoguchi H et al. Intranephron localization and regulation of the V1a vasopressin receptor during chronic metabolic acidosis and dehydration in rats. Pflugers Arch 2001; 442 (5): 652-661Sarmiento JM, Ehrenfeld P, Aсazco CC et al. Differential distribution of the vasopressin V receptor along the rat nephron during renal ontogeny and maturation. Kidney Int 2005; 68 (2): 487-796Sarmiento JM, Ehrenfeld P, Aсazco CC et al. Differential distribution of the vasopressin V receptor along the rat nephron during renal ontogeny and maturation. Kidney Int 2005; 68 (2): 487-796Arpin-Bott MP, Kaissling B, Waltisperger E et al. Historadioautographic localization of oxytocin and V1a vasopressin binding sites in the kidney of developing and adult rabbit, mouse and merione and of adult human. Exp Nephrol 2002; 10 (3): 196-208Arpin-Bott MP, Kaissling B, Waltisperger E et al. Historadioautographic localization of oxytocin and V1a vasopressin binding sites in the kidney of developing and adult rabbit, mouse and merione and of adult human. Exp Nephrol 2002; 10 (3): 196-208Gonzalez CB, Figueroa CD, Reyes CE et al. Immunolocalization of V1 vasopressin receptors in the rat kidney using anti-receptor antibodies. Kidney Int 1997; 52 (5):1206-1215Gonzalez CB, Figueroa CD, Reyes CE et al. Immunolocalization of V1 vasopressin receptors in the rat kidney using anti-receptor antibodies. Kidney Int 1997; 52 (5):1206-1215Burnatowska-Hledin MA, Spielman WS. Vasopressin V1 receptors on the principal cells of the rabbit cortical collecting tubule. Stimulation of cytosolic free calcium and inositol phosphate production via coupling to a pertussis toxin substrate. J Clin Invest 1989; 83 (1): 84–89Burnatowska-Hledin MA, Spielman WS. Vasopressin V1 receptors on the principal cells of the rabbit cortical collecting tubule. Stimulation of cytosolic free calcium and inositol phosphate production via coupling to a pertussis toxin substrate. J Clin Invest 1989; 83 (1): 84–89Carmosino M, Brooks HL, Cai Q et al. Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct. Am J Physiol Renal Physiol 2006; 292 (1): F351-F360Carmosino M, Brooks HL, Cai Q et al. Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct. Am J Physiol Renal Physiol 2006; 292 (1): F351-F360Mutig K, Paliege A, Kahl T et al. Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL. Am J Physiol Renal Physiol2007; 293 (4): F1166-F1177Mutig K, Paliege A, Kahl T et al. Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL. Am J Physiol Renal Physiol2007; 293 (4): F1166-F1177Сыромятников ДБ. Биохимические нормы в педиатрии. Сотис, СПб., 1996, 94Сыромятников ДБ. Биохимические нормы в педиатрии. Сотис, СПб., 1996, 94Ostrowski NL, Young 3d WS, Knepper MA, Lolait SJ. Expression of vasopressin V1a and V2 receptor messenger ribonucleic acid in the liver and kidney of embryonic, developing, and adult rats. Endocrinology 1993; 133 (4), 1849-1859Ostrowski NL, Young 3d WS, Knepper MA, Lolait SJ. Expression of vasopressin V1a and V2 receptor messenger ribonucleic acid in the liver and kidney of embryonic, developing, and adult rats. Endocrinology 1993; 133 (4), 1849-1859Terada Y, Tomita K, Nonoguchi H, Yang T, Marumo F. Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction. J Clin Invest 1993; 92 (5): 2339-2345Terada Y, Tomita K, Nonoguchi H, Yang T, Marumo F. Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction. J Clin Invest 1993; 92 (5): 2339-2345Robben JH, Knoers NVAM, Deen PMT. Regulation of the vasopressin V2 receptor by vasopressin in polarized renal collecting duct cells. Mol Biol Cell 2004; 15 (12): 5693-5699Robben JH, Knoers NVAM, Deen PMT. Regulation of the vasopressin V2 receptor by vasopressin in polarized renal collecting duct cells. Mol Biol Cell 2004; 15 (12): 5693-5699Fenton RA, Brшnd L, Nielsen S, Praetorius J. Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney. Am J Physiol Renal Physiol 2007; 293 (3): F748-F760Fenton RA, Brшnd L, Nielsen S, Praetorius J. Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney. Am J Physiol Renal Physiol 2007; 293 (3): F748-F760Knepper M, Hoffert J, Packer R, Fenton R. Urine concentration and dilution. In: Brenner BM, ed. Brenner and Rector’s. The Kidney. 9nd ed. WB Saunders, Philadelphia, 2008; 308-329Knepper M, Hoffert J, Packer R, Fenton R. Urine concentration and dilution. In: Brenner BM, ed. Brenner and Rector’s. The Kidney. 9nd ed. WB Saunders, Philadelphia, 2008; 308-329Morel F. Sites of hormone action in the mammalian nephron. Am J Physiol 1981; 240 (3): F159-F164Morel F. Sites of hormone action in the mammalian nephron. Am J Physiol 1981; 240 (3): F159-F164Trinder D, Phillips PA, Stephenson JM et al. Vasopressin V1 and V2 receptors in diabetes mellitus. Am J Physiol Endocrinol Metab 1994; 266: E217-E223Trinder D, Phillips PA, Stephenson JM et al. Vasopressin V1 and V2 receptors in diabetes mellitus. Am J Physiol Endocrinol Metab 1994; 266: E217-E223Goncharevskaya OA, Shakhmatova EI, Natochin YV. Modulation of the effect of arginine-vasopressin on water and ion transport in the newt early distal tubule and frog urinary bladder by V1-antagonists. Eur J Physiol 1995; 430: 1004-1011Goncharevskaya OA, Shakhmatova EI, Natochin YV. Modulation of the effect of arginine-vasopressin on water and ion transport in the newt early distal tubule and frog urinary bladder by V1-antagonists. Eur J Physiol 1995; 430: 1004-1011Birnbaumer M, Antaramian A, Themmen AP, Gilbert S. Desensitization of the human V2 vasopressin receptor. Homologous effects in the absence of heterologous desensitization. J Biol Chem 1992; 267 (17): 11783-11788Birnbaumer M, Antaramian A, Themmen AP, Gilbert S. Desensitization of the human V2 vasopressin receptor. Homologous effects in the absence of heterologous desensitization. J Biol Chem 1992; 267 (17): 11783-11788Bankir L, Fernandes S, Bardoux P et al. Vasopressin-V2 receptor stimulation reduces sodium excretion in healthy humans. J Am Soc Nephrol 2005; 16 (7):1920-1928Bankir L, Fernandes S, Bardoux P et al. Vasopressin-V2 receptor stimulation reduces sodium excretion in healthy humans. J Am Soc Nephrol 2005; 16 (7):1920-1928Cowley AW Jr. Control of the renal medullary circulation by vasopressin V1a and V2 receptors in the rat. Exp Physiol 2000; 85: 223S-231SCowley AW Jr. Control of the renal medullary circulation by vasopressin V1a and V2 receptors in the rat. Exp Physiol 2000; 85: 223S-231SMasilamani S, Knepper MA, Burg MB. Urineconcentration and delution. In Brenner BM, ed.: The kidney, 6th ed. Philadelphia, WB Saunders, 2000; 595-635Masilamani S, Knepper MA, Burg MB. Urineconcentration and delution. In Brenner BM, ed.: The kidney, 6th ed. Philadelphia, WB Saunders, 2000; 595-635Наточин ЮВ Клиническая физиология почек у детей. В: Папаян АВ, Савенкова НД, ред. Клиническая нефрология детского возраста. Левша, СПб., 2008; 19-56Наточин ЮВ Клиническая физиология почек у детей. В: Папаян АВ, Савенкова НД, ред. Клиническая нефрология детского возраста. Левша, СПб., 2008; 19-56Кузнецова АА, Наточин ЮВ. Исследование развития функций почек и их регуляции у детей на фоне патологического процесса. Журн эвол биохим и физиол 1995; 41 (3): 277-284Кузнецова АА, Наточин ЮВ. Исследование развития функций почек и их регуляции у детей на фоне патологического процесса. Журн эвол биохим и физиол 1995; 41 (3): 277-284Тареева ИЕ, Игнатова МС. Канальцевые дисфункции. В: Тареева ИЕ, ред. Нефрология: руководство для врачей. Медицина, М., 2000; 320-328Тареева ИЕ, Игнатова МС. Канальцевые дисфункции. В: Тареева ИЕ, ред. Нефрология: руководство для врачей. Медицина, М., 2000; 320-328

The authors declare that there are no conflicts of interest present.