nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2017-21-1-57-67nefr-224Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. EXPERIMENTAL INVESTIGATIONВЛИЯНИЕ КОМБИНИРОВАННОГО ВВЕДЕНИЯ КРЫСАМ ТИРОКСИНА И ПРОПИЛТИОУРАЦИЛА НА СТРУКТУРНЫЕ ПОКАЗАТЕЛИ ПОЧЕЧНОЙ ПАРЕНХИМЫINFLUENCE OF COMBINED INJECTION OF THYROXIN AND PROPYLTHIOURACILUM ON SCTRUCTURAL INDICATORS OF RENAL PARENCHYMAДоломатовС. И.DolomatovS. I.

Доцент Доломатов Сергей Игоревич, кандидат биологических наук

Кафедра биологии медицинской

295006, Россия, г. Симферополь, бул. Ленина, д. 5/7

Associate prof Sergey I. Dolomatov MD, PhD. 

295006, Russia, Simferopol, Lenin boulevard, 5/7. 

path888d@yandex.ruСиповскийВ. Г.SipovskiV. G.

Доцент Сиповский Василий Георгиевич, кандидат медицинских наук 

Научно-исследовательский институт нефрологии 

197089, Россия, Санкт-Петербург, ул. Льва Толстого, д. 17

Associate prof. Vassili G. Sipovski MD, PhD. 

Research Institute of Nephrology 

197022, Russia, Saint-Petersburg, Str. Leo Tolstoy, 17

НовиковН. Ю.NovikovN. Y.

Новиков Николай Юлиевич, доктор медицинских наук, старший научный сотрудник.

Senior Researcher Nikolay J. Novikov MD, PhD, DMedSci.

249031, Russia, Obninsk, Marshal Zhukov str. 10. 

КасичИ. Н.KasichI. N.

Касич Игорь Николаевич - студент

Student Igor N. Kasich

МышкоИ. В.MyshkoI. V.

Мышко Илья Владимирович - студент

Student Ilya V. Myshko

ДериК.DeriК.

Дери Карим - студент

Student Karim Deri

ЛитвиненкоА. Н.LytvinenkoA. N.

Литвиненко Антон Николаевич 

350911, Россия, г. Краснодар, ул. Садовая, д. 9. 

Anton N. Lytvinenko MD. 

350911, Russia, Krasnodar, Sadovaya str. 9. 

Медицинская академия им. С.И. Георгиевского, Крымского Федерального университета им. В.И. ВернадскогоРоссияMedical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal UniversityRussian FederationПервый Санкт-Петербургский государственный медицинский университет им. акад. И.П. ПавловаРоссияFirst Pavlov Saint Petersburg State Medical UniversityRussian FederationМедицинский радиологический научный центр им. А.Ф. Цыба, филиал Национального медицинского исследовательского радиологического центраРоссияA. Tsyba Medical Radiological Research Centre – branch of the National Medical Research Radiological CentreRussian FederationМедицинская академия им. С.И. Георгиевского Крымского Федерального университета им. В.И. Вернадского.РоссияMedical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal UniversityRussian FederationГородская поликлиника №11.РоссияPolyclinic №11Russian Federation2017030320172115767Copyright © Доломатов С.И., Сиповский В.Г., Новиков Н.Ю., Касич И.Н., Мышко И.В., Дери К., Литвиненко А.Н., 20172017Доломатов С.И., Сиповский В.Г., Новиков Н.Ю., Касич И.Н., Мышко И.В., Дери К., Литвиненко А.Н.Dolomatov S.I., Sipovski V.G., Novikov N.Y., Kasich I.N., Myshko I.V., Deri К., Lytvinenko A.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/224

ЦЕЛЬ РАБОТЫ – изучение динамики структурных изменений ренальной паренхимы крыс, подвергавшихся длительному комбинированному воздействию тироксина и ПТУ. МАТЕРИАЛЫ И МЕТОДЫ. Исследования проведены на беспородных самцах белых крыс массой тела 250–300 г. Гипертиреоз вызывали ежедневным внутрижелудочным введением тироксина (Т4) в количестве 50 мкг на100 гмассы тела на протяжении 30 сут. В первый день эксперимента животные были разделены на 2 группы. Животные первой группы (n=25) получали только Т4. Крысам второй группы (n=25) ежедневно вводили Т4+пропилтиоурацил (ПТУ). ПТУ вводили внутрижелудочно по 1 мг на100 гмассы тела. Пробы тканей почек собирали на 10-, 20-е и 30-е сутки эксперимента. Кроме того, был проведен сбор образцов ткани почек животных, получавших только Т4 через 20 сут после прекращения введения гормона. Полученные пробы ткани фиксировали и обрабатывали по общепринятой методике с последующей заливкой в парафин. Срезы окрашивали гематоксилином и эозином. РЕЗУЛЬТАТЫ. Установлено, что течение экспериментального гипертиреоза приводит к существенным структурным нарушениям почечной паренхимы. Ведущие проявления патологии почек при гипертиреозе – это грубые структурные повреждения канальцевого эпителия нефрона. ЗАКЛЮЧЕНИЕ. Комбинированное введение крысам тироксина и пропилтиоурацила дает слабо выраженный благоприятный эффект, ограничивая развитие структурных повреждений ренальной паренхимы и образования тромбов. 

THE AIM: to study of the dynamics of structural changes in renal parenchyma of rats exposed to long-term combined effects of thyroxine and propylthiouracilum (PTU). MATHERIAL AND METHODS – studies were performed on mongrel white male rats weighing 250-300g. Hyperthyroidism was caused by daily intragastric administration of thyroxine (T4) in amount of 50g per 100g of body weight over 30 days. On the first day of the experiment animals were divided into 2 groups. Animals of the first group (n = 25) received only T4. The rats of the second group (n = 25) were administrated propylthiouracilum and T4 daily. PTU was administered intragastric in amount of 1 mg per 100g of body weight. Kidney tissue samples were collected on the 10th, 20th and 30th days of the experiment. In addition, there were collected kidney tissue samples of the animals treated with only T4 after 20 days after cessation of hormone. Obtained tissue samples were fixed and treated by the usual method, followed by filling in paraffin. Sections were stained with hematoxylin and eosin. RESULTS – it was established that course of experimental hyperthyroidism leads to significant structural abnormalities of the renal parenchyma. Leading features of kidneys pathology at a hyperthyroidism are rough structural damages of the nephron tubular epithelium. CONCLUSIONS – combined administration in rats of thyroxin and propylthiouracilum has weakly expressed beneficial effect by limiting the development of structural damages to the renal parenchyma and clot formation. 

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The authors declare that there are no conflicts of interest present.