nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical Universitynefr-231Research ArticleПЕРЕДОВАЯ СТАТЬЯLEADING ARTICLEРЕГЕНЕРАТИВНЫЕ СТРАТЕГИИ РЕКОНСТРУИРОВАНИЯ ПОЧКИREGENERATIVE STRATEGIES FOR KIDNEY ENGINEERINGМонтсерратН.MontserratN.

Программы по плюрипотентным стволовым клеткам и активации эндогенной ткани для регенерации органов (PR Lab)

Pluripotent Stem Cells and Activation of Endogenous Tissue Programs for Organ Regeneration (PR Lab)

ГарретаЕ.GarretaE.

Программы по плюрипотентным стволовым клеткам и активации эндогенной ткани для регенерации органов (PR Lab)

Pluripotent Stem Cells and Activation of Endogenous Tissue Programs for Organ Regeneration (PR Lab)

БельмонтеХ.К.И.BelmonteJ.C.I.

Лаборатория экспрессии гена

Gene Expression Laboratory

Институт Биоинжиниринга Каталонии (IBEC); Сетевой биомедицинский исследовательский центр биоинжиниринга, биоматериалов и наномедицины (CIBER-BBN)ИспанияInstitute for Bioengineering of Catalonia (IBEC); Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), MadridSpainИнститут Биоинжиниринга Каталонии (IBEC);ИспанияInstitute for Bioengineering of Catalonia (IBEC)SpainИнститут биологических исследований СолкаСоединённые Штаты АмерикиSalk Institute for Biological Studies, La Jolla, CAUnited States2016030320172061025Copyright © Монтсеррат Н., Гаррета Е., Бельмонте Х., 20172017Монтсеррат Н., Гаррета Е., Бельмонте Х.Montserrat N., Garreta E., Belmonte J.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/231

Почка является важнейшим органом водного гомеостаза и экскреции токсических субстанций. Она выполняет несколько важных физиологических функций для обеспечения гомеостаза: удаляет циркулирующие продукты метаболизма, регулирует баланс жидкости в организме и действует как иммунный регулятор, а также модулятор нормального функционирования сердечно-сосудистой системы. В самое последнее время появились и активно развиваются модели почечных заболеваний in vitro с плюрипотентными стволовыми клетками (как стволовыми клетками человеческого эмбриона, так и индуцированными плюрипотентными стволовыми клетками), а также разрабатываются надежные протоколы по получению in vitro клеток, подобных специфичным ренальным, из индуцированных плюрипотентных стволовых клеток пациента. В данном обзоре мы приводим главные открытия в области регенерации почки с основным фокусом на развитие пошаговых протоколов по созданию почечных клеток из человеческих плюрипотентных стволовых клеток и самые последние достижения в области биоинжиниринга почки (т.е. децеллюляризированного почечного остова и биопринтинга). Возможность создания трехмерной структуры, подобной почке, с последующим наполнением ее индуцированными плюрипотентными стволовыми клетками почечного происхождения может открыть новые перспективы для создания функционирующего по требованию почечного трансплантата.

Статья переведена на русский язык и опубликована согласно условиям лицензии Creative Commons. Журнал FEBS J. 2016 Sep;283(18):3303–24. doi: 10.1111/febs.13704. Перевод М.С.Храбровой. Редакция перевода И.И.Трофименко

The kidney is the most important organ for water homeostasis and waste excretion. It performs several important physiological functions for homeostasis: it filters the metabolic waste out of circulation, regulates body fluid balances, and acts as an immune regulator and modulator of cardiovascular physiology. The development of in vitro renal disease models with pluripotent stem cells (both human embryonic stem cells and induced pluripotent stem cells) and the generation of robust protocols for in vitro derivation of renal-specific-like cells from patient induced pluripotent stem cells have just emerged. Here we review major findings in the field of kidney regeneration with a major focus on the development of stepwise protocols for kidney cell production from human pluripotent stem cells and the latest advances in kidney bioengineering (i.e. decellularized kidney scaffolds and bioprinting). The possibility of generating renal-like three-dimensional structures to be recellularized with renal-derived induced pluripotent stem cells may offer new avenues to develop functional kidney grafts on-demand.

индуцированные плюрипотентные стволовые клеткизаболевания почекреконструкция почекплюрипотентные стволовые клеткипочечно-клеточная дифференциацияinduced pluripotent stem cellskidney diseasekidney engineeringpluripotent stem cellsrenal differentiationM. Schwarz, P. Schwarz за административную помощь. N.M. был частично поддержан StG-2014-640525_REGMAMKID, RyC 2014-16242, Fundaci_o Privada La Marat_o de TV3 (121430/31/32) Министерством экономики и конкурентноспособности Испании (SAF2014-59778) и комиссией по университетам и научным исследованиям отдела инноваций, университетов и предприятий генералитата Каталонии (2014 SGR 1442). E.G. был частично поддержан StG-2014-640525_REGMAMKID and La Fundaci_o Privada La Marat_o de TV3, 121430/31/32. J.C.I.B. был поддержан грантами фонда Glenn, благотворительного фонда G.Harold и Leila Y.Mathers и благотворительный фонд.Leona M. and Harry B. 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The authors declare that there are no conflicts of interest present.