nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2006-10-1-45-49nefr-437Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. КЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. CLINICAL INVESTIGATIONSУЧАСТИЕ КРОВЕТВОРНЫХ СТВОЛОВЫХ КЛЕТОК В РЕГЕНЕРАЦИИ ПОЧКИ ПРИ ХРОНИЧЕСКОМ ГЛОМЕРУЛОНЕФРИТЕINVOLVEMENT OF HEMOPOIETIC STEM CELLS IN REGENERATION OF THE KIDNEY IN CHRONIC GLOMERULONEPHRITISСметанниковаТ. С.SmetannikovaT. S.

кафедра нормальной анатомии

ГумероваА. А.GumerovaA. A.

кафедра нормальной анатомии

ХайрулловА. С.KhajrullovA. S.

кафедра нормальной анатомии

КиясовА. П.KiyasovA. P.

кафедра нормальной анатомии

Казанский государственный медицинский университет, ТатарстанRussian Federation2006100120061014549Copyright © Сметанникова Т.С., Гумерова А.А., Хайруллов А.С., Киясов А.П., 20062006Сметанникова Т.С., Гумерова А.А., Хайруллов А.С., Киясов А.П.Smetannikova T.S., Gumerova A.A., Khajrullov A.S., Kiyasov A.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/437

ЦЕЛЬ ИССЛЕДОВАНИЯ: изучить возможность участия кроветворных стволовых клеток в регенерации почки при хроническом гломерулонефрите (ХГН) с различной степенью морфологической активности ХГН. МАТЕРИАЛ И МЕТОДЫ. Исследования проведены на материале 64 нефробиопсий больных ХГН. Патоморфологическую оценку активности ХГН и выраженности (склероза) осуществляли полуколичественным методом. Иммуногистохимическое исследование нефробиоптатов проводили с использованием моноклональных антител против α-ГМА, CD31, CD34 стрептовидинбиотиновым методом (LSAB-kit компании, DAKO). α-ГМК-актин-маркер миофибробластов, синтезирующих компоненты соединительной ткани. СD31-маркер эндотелия, CD34 – эндотелия и гемопоэтических стволовых клеток. РЕЗУЛЬТАТЫ. При минимальной степени активности ХГН, количество клеток, экспрессирующих СD34 в 18,75% случаев превышало количество клеток, экспрессирующих CD31. Экспрессия α-ГМА миофибробластам выявлена в 28%. При умеренной степени активности повышенная экспрессия CD34 встречалася в 46,15% случаев. Миофибробласты α-ГМА+ выявлены во всех нефробиопсиях. При средней активности ХГН клеточная экспрессия CD34 превышала CD31 в 50%. При высокой степени активности уровень экспрессии СD34 был равен уровню экспрессии СD31. Возможное участие в регенераторном ответе кроветворных стволовых клеток определяли по разнице экспрессии CD31 и CD34. ЗАКЛЮЧЕНИЕ. Кроветворные стволовые клетки с фенотипом CD34+ могут принимать участие в регенерации почки при ХГН.

THE AIM of the investigation was to study possible involvement of hemopoietic stem cells in regeneration of the kidney in chronic glomerulonephritis (CGN) with different degrees of morphological activity of CGN. MATERIALS AND METHODS. The investigations were performed with the material of 64 nephrobiopcies of CGN patients. Pathomorphological assessment of CGN activity and degree of sclerosis was fulfilled by a semiquantitative method. The immunohistochemical investigation of nephrobioptates was carried on using monoclonal antibodies against α-smooth muscle actin (α-SMA), CD31, CD34 streptovidinbiotin method (LSAB-kit company, DAKO), α-SMC-actin marker of myofibroblasts synthesizing components of the connective tissue, CD31-marker of endothelium, CD34 – of the endothelium and hemopoietic stem cells. RESULTS.The number of cells expressing CD34 in 18.75% of cases under condition of minimal degree of CGN activity exceeded the number of cells expressing CD31. Expression of α-SMA by myofibroblasts was detected in 28%. In cases of mild degree of activity the higher expression of CD34 took place in 46.15% of cases. Myofibroblasts of α-SMA+ were found in all nephrobiopcies. In average activity of CGN the cell expression of CD34 was 50% higher than CD31. In high degree of activity the level of CD34 expression was equal to that of CD31. Possible participation in the regenerative response of hemopoietic stem cells was determined by difference of expression of CD31 and CD34. CONCLUSION. Hemopoietic stem cells with phenotype CD34+ can be involved in regeneration of the kidney in CGN.

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The authors declare that there are no conflicts of interest present.