nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2007-11-1-7-16nefr-737Research ArticleПЕРЕДОВЫЕ СТАТЬИLEADING ARTICLEАККУМУЛЯЦИЯ В ПОЧКЕ ЭКЗОГЕННОГО БЕЛКА ПОСЛЕ ЕГО ВСАСЫВАНИЯ В КИШЕЧНИКЕ ПРИ РАЗВИТИИ ЭКСПЕРИМЕНТАЛЬНОЙ ПОЧЕЧНОЙ НЕДОСТАТОЧНОСТИ У КРЫСACCUMULATION IN THE KIDNEY OF EXOGENOUS PROTEIN AFTER ITS ABSORPTION IN THE INTESTINE DURING THE DEVELOPMENT OF EXPERIMENTAL RENAL FAILURE IN RATSСеливерстоваЕ. В.SeliverstovaE. V.БурмакинМ. В.BurmakinM. V.ШахматоваЕ. И.ShakhmatovaE. I.СмирновА. В.SmirnovA. V.ДобронравовВ. А.DobronravovV. A.СиповскийВ. Г.SipovskyV. G.БересневаО. Н.BeresnevaO. N.ПарастаеваМ. М.ParastaevaM. M.КанашкинаТ. А.KanashkinaT. A.МнускинаМ. М.MnuskinaM. M.НаточинЮ. В.NatochinYu. V.Институт эволюционной физиологии и биохимии им. И.М. Сеченова РАН; Научно-исследовательский институт нефрологии Cанкт-Петербургского государственного медицинского университета имени акад. И.П. ПавловаRussian Federation200710012007111716Copyright © Селиверстова Е.В., Бурмакин М.В., Шахматова Е.И., Смирнов А.В., Добронравов В.А., Сиповский В.Г., Береснева О.Н., Парастаева М.М., Канашкина Т.А., Мнускина М.М., Наточин Ю.В., 20072007Селиверстова Е.В., Бурмакин М.В., Шахматова Е.И., Смирнов А.В., Добронравов В.А., Сиповский В.Г., Береснева О.Н., Парастаева М.М., Канашкина Т.А., Мнускина М.М., Наточин Ю.В.Seliverstova E.V., Burmakin M.V., Shakhmatova E.I., Smirnov A.V., Dobronravov V.A., Sipovsky V.G., Beresneva O.N., Parastaeva M.M., Kanashkina T.A., Mnuskina M.M., Natochin Y.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/737

ЦЕЛЬ ИССЛЕДОВАНИЯ – изучение возможности накопления в почке крыс зеленого флюоресцентного белка (ЗФБ) в условиях экспериментальной хронической почечной недостаточности (ХПН). МАТЕРИАЛ И МЕТОДЫ. Экспериментальную группу составили крысы линии Вистар (n=13), подвергнувшиеся субтотальной нефрэктомии (СНЭ), через 8 недель после которой животным через желудочный зонд вводили ЗФБ. В качестве контрольной группы использовали животных с интактными почками (n=12). В обеих группах определяли концентрацию креатинина, мочевины плазмы крови, суточный диурез, концентрацию альбумина и креатинина мочи с расчетом отношения альбумин/креатинин (ACR), концентрационного индекса (Ucr/Pcr) и клиренса креатинина (Ccr), а также проводили светооптическое и электронномикроскопическое исследование почек. Для оценки накопления ЗФБ в энтероцитах и клеточных популяциях нефрона применяли конфокальную микроскопию. РЕЗУЛЬТАТЫ. Установлено, что ЗФБ всасывается энтероцитами и, поступая в системную циркуляцию, накапливается в везикулах клеток проксимального канальца (ПК). Морфологические и функциональные изменения у крыс с развивающейся почечной недостаточностью после СНЭ сопровождается значительным снижением накопления ЗФБ в ПК в сравнении с животными без дисфункции почек. ЗАКЛЮЧЕНИЕ. На примере модели с ЗФБ у животных с экспериментальной ХПН впервые продемонстрирована принципиальная возможность энтерального транспорта и накопления в клетках проксимального сегмента нефрона интактных чужеродных белков. Полученные данные открывают новые перспективы для изучения роли почек в метаболизме экзогенных протеинов.

THE AIM of the investigation was to study the possibilities of accumulation of the green fluorescent protein (GFP) in the rat kidney under conditions of experimental chronic renal failure (CRF). MATERIALS AND METHODS. The experimental group consisted of 13 Wistar rats subjected to subtotal nephrectomy (SNE). In eight days after operation GFP was introduced to the animals through stomach probe. The control group consisted of 12 rats with intact kidneys. The concentration of creatinine, blood plasma urea, diurnal diuresis, concentration of albumin and urine creatinine with calculation of the albumine/cratinine ratio (ACR), concentration index (Ucr/Pcr) and creatinine clearance (Ccr ) were determined in both groups. In addition, light-optical and electron-microscopic investigations were carried on. Confocal microscopy was used for the assessment of accumulation of GFP in the erythrocytes andcell populations of the nephron. RESULTS. It has been found that GFP is absorbed by erythrocytes and, on coming into the systemic circulation, is accumulated in the cell vesicles of the proximal tubules (PT). Morphological and functional changes in the rats with the developing renal failure after SNE are followed by a considerable decrease of accumulation of GFP in PT as compared with the animals without dysfunction of the kidneys. CONCLUSION. The example of the model with GFP in animals with experimental CRF for the first time has demonstrated the fundamental possibility of enteral transport and accumulation of intact foreign proteins in the cells of the proximal nephron segment. The data obtained open new perspectives for studying the role of the kidneys in metabolism of exogenous proteins.

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The authors declare that there are no conflicts of interest present.