nefrНефрологияNephrology (Saint-Petersburg)1561-62742541-9439Pavlov First Saint-Petersburg State Medical University10.24884/1561-6274-2003-7-3-60-66nefr-935Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИ. ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLES. EXPERIMENTAL INVESTIGATIONВЛИЯНИЕ ИНГИБИТОРОВ АЛКОГОЛЬДЕГИДРОГЕНАЗЫ НА БИОХИМИЧЕСКИЕ И ГИСТОЛОГИЧЕСКИЕ ИЗМЕНЕНИЯ ПРИ ЭКСПЕРИМЕНТАЛЬНЫХ ОТРАВЛЕНИЯХ ЭТИЛЕНГЛИКОЛЕМ И ЕГО ЭФИРАМИEFFECTS OF ALCOHOL DEHYDROGENASE INHIBITORS ON BIOCHEMICAL AND HISTOLOGICAL CHANGES IN EXPERIMENTAL POISONINGS WITH ETHYLENE GLYCOL AND ITS ETHERSБонитенкоЕ. Ю.BonitenkoE. Yu.БабаханянР. В.BabakhanyanR. V.ЕсаянА. М.EssaianA. M.Санкт-Петербургский государственный медицинский университет им. акад. И.П.ПавловаRussian Federation200310032003736066Copyright © Бонитенко Е.Ю., Бабаханян Р.В., Есаян А.М., 20032003Бонитенко Е.Ю., Бабаханян Р.В., Есаян А.М.Bonitenko E.Y., Babakhanyan R.V., Essaian A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.nephrolog.ru/jour/article/view/935

ЦЕЛЬ ИССЛЕДОВАНИЯ -изучение влияния ингибиторов алкогольдегидрогеназы (АДГ), амида изовалериановой кислоты (АИК) и этанола на основные биохимические показатели и гистологические изменения при острых пероральных отравлениях этиленгликолем (ЭГ) и его метиловым и этиловым эфирами. МАТЕРИАЛ И МЕТОДЫ. Работа выполнена на 240 беспородных белых крысах-самцах массой 140 -180 г разводки питомника Рапполово. ЭГ и его монометиловый (метилцеллозольв; МЦ) и моноэтиловый (этилцеллозольв; ЭЦ) эфиры вводились однократно внутрижелудочно в дозе, равной 1,ОЛД 50 (6928 ± 352,1761 ± 204, 2643 ± 316 мг/кг соответственно). В качестве ингибиторов АДГ применяли бесконкурентный ингибитор АИК, а для субстратного ингибирования - этиловый спирт. РЕЗУЛЬТАТЫ. Было установлено, что при интоксикациях этими веществами биохимические изменения носят схожий характер и отличаются не качественно, а количественно. Наиболее выраженными они были при интоксикациях ЭП а ведущими среди них являлись изменения, характеризующие поражение почек (повышение концентрации азотистых шлаков) и печени (увеличение активности аминотрансфераз, щелочной фосфатазы и концентрации билирубина). Гистологические изменения при отравлениях ЭГ и его эфирами также носили сходный характер и были более выражены при интоксикациях гликолем. Применение этанола и АИК значительно уменьшало выраженность как биохимических, так и гистологических изменений, вызываемых ЭГ и его эфирами. ЗАКЛЮЧЕНИЕ. Полученные нами данные позволяют говорить о том, что перспективным направлением совершенствования антидотной терапии этих интоксикаций является использование специфических ингибиторов АДГ.

THEAIM of the work was to investigate the effects of alcohol dehydrogenase (ADG) inhibitors, isovalerianic acid amid (IAA) and ethanol on main biochemical indices and histological alterations in acute peroral poisonings with ethylene glycol (EG) and its methyl and ethyl ethers. MATERIAL AND METHODS. Th ewor kwas fulfilled in 240 white male rats (body mass 140-180 g) got from animal nursery Rappolovo. EG and its monomethyl (methyl cellozolv)and monoethyl (ethyl cellozolv) ethers were given as a single intragastric dose equal to 1.0 LD50 (6928±352,11761 ±204,2643±316 mg/mg respectively). A noncompetitive inhibitor of IAA was used as ADG inhibitors, and ethyl alcohol - for the substrate inhibition. RESULTS. It was found that intoxications with these substances result in biochemical changes of a similar character and differ not qualitatively but quantitatively. They were more pronounced in EG intoxications, the leading among them being changes showing lesion of the kidneys (higher concentration of nitrogenous metabolites) and liver (increased activity of aminotransferases, alkaline phosphatase and concentration of bilirubin). The histological alterations caused by poisonings with EG and its ethers were of a similar character and were more pronounced in intoxications with glycol. The administration of ethanol and IAA considerably decreased the degree of both biochemical and histological changes caused by EG and its ethers. CONCLUSION. The data obtained suggest that using specific ADG inhibitors is a perspective direction of the improvement of antidotal therapy of such intoxications.

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The authors declare that there are no conflicts of interest present.