THE INFLUENCE OF FITOCOMPLEXES OF THE CELL CULTURE OF RUBIA CORDIFOLIA ON THE DEVELOPMENT OF EXPERIMENTAL NEPHROLITHIASIS IN RATS

THE AIM of the investigation was to study the influence of the long term introduction of fitocomplexes of th cell culture of Rubia cordifolia on the renal function of rats in the conditions of experimental oxalate nephrolithiasis. MATERIALS AND METHODS. For the modeling of the kidney stone disease the male rats Wistar during the three weeks received as drink water 1% solution of ethilenglicol. The prophylactic effect of fitocomplexes of Rubia Cordifoliawere evaluated in conditions of simultaneous injection of the cell culture in the dose of 75 mg/kg in suspension based on starch mucus. In the urine test, collected during 24 hours, the concentration of oxalate-, phosphate-ions and calcium ions, and also the activity of lactetdehydrogenase enzime, gamma-glutaminiltransferase and N-acetyl-β-D-glucoseaminidase were evaluated. RESULTS. Under the influence of ethyleneglycol the oxalate nephrolithiasis developed in rats, with was accompanied by the increase of the oxalate excretion and the development of the fermenturia. In the conditions of prophylactic use of the fitocomplexes of the cell cultures of the Rubia Cardifoliawas noted the decrease in oxalate excretion in 2,6 times in comparison with the rats with experimental nephrolithiasis. During the use of this fito-medicine the stabilization of the activity of marker enzimes, especially lactatdehydrogenase and gamma glutamiltransferase was registered. CONCLUSION. The good influence of the prophylactic use of fitocomplexes of th cell culture of Rubia Cordifoliaon the flow of the experimental kidney stone disease was realized in lower development of oxaluria and stabilization of the activity of the urine marker ferments.

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