THE ROLE OF ANTIOXIDANT THERAPY IN EXPERIMENTAL PHARMACOLOGICAL CORRECTION OF NEPHROLITHIASIS

Abstract

THE AIM. The study was undertaken to study the influence of α-tocopherol acetate, a classical anti-oxidant, in the course of experimental nephrolithiasis. MATERIAL AND METHODS. Wistar male rats for 3 weeks were given as a drink 1% solution ethylene glycol, then, within the next 3 weeks, amid continued use of ethylene glycol the oil solution of б-tocopherol acetate 300 mg / kg was injected. In the urine collected per day, measured the concentration of oxalate, calcium and phosphate, pH of urine and the activity of marker enzymes lactatdehydorgenase (LDH), γ-glutamyltransferase (GGT) and N-acetyl-β-D-glucosaminidase (NAG). On the 24-th and 42-th days of the experiment was determined by the activity rate of free radical oxidation in the rat kidney. Histochemical method by Coss has been used for determination calcium-positive deposits on kidney slices of rats. RESULTS. Application of α-tocopherol acetate caused significant relief during the trial of nephrolithiasis. To a large extent suppressed by oxidative stress, decreased to normal values of the activity of marker enzymes, more than 60% decreased the concentration in urine oxalate ions, and decreased to the level corresponding to the healthy rats, the concentration in urine of calcium ions. In addition, рН urine it was displaced in an alkaline side. These changes resulted in a significant decrease in the number and size of calcium deposits in the kidneys. CONCLUSION. As a result of experiments established the important role of antioxidant therapy in the pharmacological correction of experimental nephrolithiasis.