Successful experience of urate nephropathy modeling in rats
Abstract
THE AIM. To create model of urate nephropathy in rats. MATERIAL AND METHODS. The study was performed on 25 male Wistar rats. To form urate nephropathy rats were administered oxonium and uric acid (UA) as part of standard laboratory mixture. Using biochemical and morphological techniques were evaluated changes obtained on days 7, 14, 21. RESULTS. During the experiment was noticed sequential growth of UA concentration in the blood plasma of animals. The concentration of uric acid in the urine and excretion of urates increased significantly. At the same time in the course of the experiment were recorded signs of kidney injury: increased excretion of protein in the urine, and also activity of several enzymes in urine. Picture of kidney injury characteristic for urate nephropathy was confirmed by histological examination results: already on the 7th day in renal tubular lumen defined urate deposits. CONCLUSION. The identified functional, biochemical and morphological changes allow to certify existence of urate nephropathy in long-term inhibition of uricase in rats, which can be considered as an adequate model of the corresponding human disease.
About the Authors
V. Yu. Perfil’Ev
Altai State Medical University
Russian Federation
Russian Federation
Ya. F. Zverev
Altai State Medical University
Russian Federation
Russian Federation
V. M. Bryukhanov
Altai State Medical University
Russian Federation
Russian Federation
T. M. Cherdantceva
Altai State Medical University
Russian Federation
Russian Federation
I. P. Bobrov
Altai State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Perfil’Ev V.Yu., Zverev Ya.F., Bryukhanov V.M., Cherdantceva T.M., Bobrov I.P. Successful experience of urate nephropathy modeling in rats. Nephrology (Saint-Petersburg). 2016;20(4):93-97. (In Russ.)
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