STATE OF THE SYSTEM OF GLUTATHIONE AND LIPID PEROXIDATION IN TISSUES OF THE LIVER AND KIDNEYS OF RATS WITH ACUTE CYCLOPHOSPHAMIDE POISONING

THE AIM of the investigation was to carry out a complex study of the state of glutathione and intensity of lipid peroxidation processes in poisoning with cyclophosphamide in dose of 200 mg/kg of body mass. MATERIAL AND METHODS. Concentrations of reduced glutathione, sulfhydril groups of proteins, malonaldehyde, diene conjugates and activity of glucose-6-phosphatedehydrogenase, glutathione reductase, glutathione peroxidase, glutathione-S-transpherase, catalase were determined spectrophotometrically in the kidney and liver tissues of 50 white outbred rats. RESULTS. It was shown that this form of intoxication was followed by pronounced changes in the glutathione system in the liver and kidney tissues of poisoned animals (reduced content of reduced glutathione, impaired activity of glutathione reductase, glucose-6-phosphatedehydrogenase, glutathione reductase, glutathione-S-transferase).Causes of the appearance of the data of bio-chemical shifts, their interstitial distinctions and their role in realization of the cytotoxic action of cyclophosphamide were discussed. CONCLUSION. So, the pathogenetical role of exhaustion of the functional resources of the glutathione system was established as well as of activation of free radical processes in realization of the cytotoxic effects of alkilating preparations. 

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