RESISTANCE TO EXTERNAL ACTION OF A CHANNEL FORMER AS A CHARACTERISTIC OF CELL MEMBRANES OF HEMODIALYSIS PATIENTS
https://doi.org/10.24884/1561-6274-2007-11-1-38-54
Abstract
THE AIM of the investigation was to develop a new test allowing assessment of the bilipid layer of erythrocyte membranes in patients on regular hemodialysis in different environmental conditions and under the influence of different effectors on the membranes against the background of the action of a channel former. PATIENTS AND METHODS. Under examination there were 147 patients (78 men and 69 women, mean age 43.9+1.4 years) treated by substitution therapy with regular hemodialysis. The control group consisted of volunteers. The erythrocytes were separated from plasma by centrifugation. The sediment was washed from plasma and transferred into wells containing either model saline or autologous plasma. Nistatin (3.0-104 М) was used as a promoter of transmembrane currents of Na+ and K+. Uabain (5.60-10" M), erythropoietin and membrane active components of autologous blood plasma were taken as effectors of the erythrocyte membrane in experiments. Saponin was used as a lysing agent. Extracellular concentration of K* was determined by the method of ion-metry using ion-selective electrodes and ion-selective field transistors with photopolymerisable polyurethane membranes. The resistance of erythrocytes to an external action of the channel former was assessed by a new index - "the integral normalized outcome of K*" which was calculated for different time intervals after administration of nistatin. When determining this index the diagram area under the curve of dependence of the normalized K* concentration in time was calculated. RESULTS. Considerable differences of the integral normalized outcome of K+ were detected for the erythrocytes of healthy donors and patients with CRF treated by regular hemodialysis during the incubation of cells in the extracellular medium of different composition and exposed to effectors of different nature. Another mechanism of the interaction of uabain with plasma membrane was shown in addition to classical inhibition of Na+, К +ATPase resulting in a paradoxical membrane-stabilizing effect observed in erythrocytes of hemodialysis patients. A destabilizing effect of erythropoietin was established under its direct influence on erythrocytes. A strong membrasne-protecting effect of the components of autologous plasma was found characteristic of both the erythrocytes of healthy doors and the cells of hemodialysis patients. CONCLUSION. The integral normalized outcome of K+ is a sufficiently informative clinico-laboratory index and can be successfully used for the assessment of plasma membrane quality in patients treated by substitution therapy with regular hemodialysis.
Keywords
hemodialysis,
blood plasmo,
erythrocyte,
membranes,
channelformers,
potassium,
erythropoietine,
ionometry
About the Authors
Yu. A. Borisov
Научно-исследовательский институт нефрологии, Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова
Russian Federation
Russian Federation
E. V. Lebedeva
Научно-исследовательский институт нефрологии, Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова
Russian Federation
Russian Federation
V. N. Spiridonov
Научно-исследовательский институт нефрологии, Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова
Russian Federation
Russian Federation
E. D. Suglobova
Научно-исследовательский институт нефрологии, Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова
Russian Federation
Russian Federation
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Review
For citations:
Borisov Yu.A., Lebedeva E.V., Spiridonov V.N., Suglobova E.D. RESISTANCE TO EXTERNAL ACTION OF A CHANNEL FORMER AS A CHARACTERISTIC OF CELL MEMBRANES OF HEMODIALYSIS PATIENTS. Nephrology (Saint-Petersburg). 2007;11(1):38-54. (In Russ.) https://doi.org/10.24884/1561-6274-2007-11-1-38-54
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