SPECIFIC EFFECTS OF LOW INTENSITY LASER RADIATION AND L"ARGININE ON MYOCARDIUM ACTIVITY OF WISTAR RATS WITH THE ACQUIRED DYSFUNCTION OF ENDOTHELIUM

THE AIM of the investigation was a comparative study of effects of the low intensity laser radiation (LILR) with the wave length 632.8 nm and a substrate for synthesis of nitric oxide (NO) Larginine on mechanical activity of cardiomyocytes of control Wistar rats and Wistar rats with experimental chronic renal failure (CRF a surgical model). MATERIAL AND METHODS. Effects of LILR and L arginine on contractility of the myocardium of rats with CRF were analyzed under conditions of the isometric regimen. The surgical model of CRF is simultaneously the model of NO synthesis deficiency. The development of uremia was followed according to the biochemical indices of blood. The method of determination of the number of circulating endotheliocytes was used to determine the degree of the endothelium lesion. RESULTS. An addition of Larginine to the perfusion solution resulted in the approximately 38.0±5.2% greater force of isometric contractions of cardiomyocytes of control Wistar rats by the 10th minute of incubation. Laser irradiation resulted in an additionally increased force of contractions. An addition of Larginine against the background of effects of laser irradiation was also followed by growth of the contraction force. The total effect of HeNe laser and Larginine did not depend on the succession of their effect. In Wistar rats with experimental CRF with the urea level 13.8±3.1 mmol/l the action of Larginine on the force of isometric contractions of rats with CRF was also of the same direction. The total effect of Larginine and LILR on the myocardium of rats with CRF was 22.5 times greater than that of action on the myocardium of control rats. CONCLUSION. The investigation results have shown practically complete reestablishment of the myocardium function in rats with CRF up to the level of control rats under the influence of LILR and Larginine that speaks of the possible additional synthesis of NO in the myocardium with a greater content of the substrate in the incubation medium and the given urea level. The endocardial cells responsible for the necessary amount of NO in the myocardium are thought to be actively functioning at this period of the development of CRF.

myocardium, contractivity, Larginine, low intensity laser radiation, chronic renal failure

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