PHARMACOLOGICAL CORRECTION OF DOXORUBICIN-INDUCED OXIDATIVE STRESS IN KIDNEYS OF RATS

THE AIM of the investigation was Doxorubicin (DOX) as a widely used anthracycline antibiotic. Administration of DOX is limited due to cardio and renal toxicity because of oxidative stress. Our aim was to investigate early nephrotoxic effects of a single dose of DOX and impact of pretreatment with melatonin, glycine and unithiol. MATERIAL AND METHODS. The animals (35 rats) were divided into 5 groups. One group (Cgroup, n=7) was a control, one group (DOXgroup, n=7) received DOX (7.5 mg/kg, i.p.), one group (Mgroup, n=7) was pretreated with melatonin (1mg/kg, i.v.), one group (Ggroup, n=7) was pretreated with glycine (50 mg/kg, i.v.), one group (Ugroup, n=7) was pretreated with unithiol (5 mg/kg, i.v.).The rats were decapitated within 24 hours. The content of glutathione (GSH), glutathione reductase, NAD(P)H:quinone oxidoreductase, glutathioneStransferase and protein carbonyl group in the homogenate of renal mass were detected. RESULTS. Less concentration of GSH was detected in the renal tissue in the DOXgroup as compared with the Cgroup of rats. There were lower levels of GSH in DOXgroup as compared with Mgroup. Activity of glutathione reductase was lower in the DOXgroup than in the Cgroup. There was no difference in the content of protein carbonyl groups. Pretreatment with melatonin and glycin resulted in normalization of GSH levels. CONCLUSION. It was shown that pretreatment with melatonin and glycin reduced the DOX induced renal damage in rats by means of restoration of GSH in the renal tissue.

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