CATALASE METABOLISM IN RATS WITH CHRONIC GLOMERULONEPHRITIS
https://doi.org/10.24884/1561-6274-2004-8-3-70-73
Abstract
THE AIM of the investigation was to study catalase metabolism in experimental animals with chronic glomerulonephritis. MATERIAL AND METHODS. The work was carried out in not thoroughbred male white rats with body mass 140160 g. Experimental model of glomerulonephritis (Heimann nephritis) was induced in experimental animals by intramuscular injecting homogenate of healthy rat kidneys in Freund’s adjuvant. The biochemical indices in rats were specified in three weeks after autoimmunization of the animals. RESULTS. It was found that chronic glomerulonephritis resulting from oxidative stress was followed by a sharp drop of catalase activity. It was shown to be due to a sharp inhibition of the rate of accumulation (Ks) of enzyme protein (from 5 to 8 times) in blood, kidneys and liver of the rats with glomerulonephritis as compared with control animals and to lead to a reliable decrease of catalase concentration in those tissues. At the same time the obviously compensatory decrease of decomposition (Kd) of the fermentative protein was observed and, as a result, a longer period of its «halflife» (t1/2) which in certain degree compensated the deficit of newly synthesized molecules of catalase. CONCLUSION. It was established that the earlier found reliable drop of catalase activity in blood, kidneys and liver of rats during the development of glomerulonephritis was due to sharply decreased rate of its biosynthesis / accumulation in these tissues.
About the Authors
N. V. Kirillova
Санкт-Петербургская государственная химико-фармацевтическая академия
Russian Federation
Russian Federation
M. M. Debel
Санкт-Петербургская государственная химико-фармацевтическая академия
Russian Federation
Russian Federation
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Review
For citations:
Kirillova N.V., Debel M.M. CATALASE METABOLISM IN RATS WITH CHRONIC GLOMERULONEPHRITIS. Nephrology (Saint-Petersburg). 2004;8(3):70-73. (In Russ.) https://doi.org/10.24884/1561-6274-2004-8-3-70-73
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