EFFECTS OF ALCOHOL DEHYDROGENASE INHIBITORS ON BIOCHEMICAL AND HISTOLOGICAL CHANGES IN EXPERIMENTAL POISONINGS WITH ETHYLENE GLYCOL AND ITS ETHERS

THEAIM of the work was to investigate the effects of alcohol dehydrogenase (ADG) inhibitors, isovalerianic acid amid (IAA) and ethanol on main biochemical indices and histological alterations in acute peroral poisonings with ethylene glycol (EG) and its methyl and ethyl ethers. MATERIAL AND METHODS. Th ewor kwas fulfilled in 240 white male rats (body mass 140-180 g) got from animal nursery Rappolovo. EG and its monomethyl (methyl cellozolv)and monoethyl (ethyl cellozolv) ethers were given as a single intragastric dose equal to 1.0 LD50 (6928±352,11761 ±204,2643±316 mg/mg respectively). A noncompetitive inhibitor of IAA was used as ADG inhibitors, and ethyl alcohol - for the substrate inhibition. RESULTS. It was found that intoxications with these substances result in biochemical changes of a similar character and differ not qualitatively but quantitatively. They were more pronounced in EG intoxications, the leading among them being changes showing lesion of the kidneys (higher concentration of nitrogenous metabolites) and liver (increased activity of aminotransferases, alkaline phosphatase and concentration of bilirubin). The histological alterations caused by poisonings with EG and its ethers were of a similar character and were more pronounced in intoxications with glycol. The administration of ethanol and IAA considerably decreased the degree of both biochemical and histological changes caused by EG and its ethers. CONCLUSION. The data obtained suggest that using specific ADG inhibitors is a perspective direction of the improvement of antidotal therapy of such intoxications.

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