THE AMOUNT OF CATIONIC PROTEINS OF NEUTROPHILS AND EXPRESSION ON THEIR MEMBRANE OF ERYTHROCYTE, COMPLEMENT AND Fcy-RECEPTORS INTYPE I MEMBRANOUS-PROLIFERATIVE GLOMERULONEPHRITIS

THE AIM the investigation was to study the degree of correlation of quantitative and functional indices of neutrophil granulocytes (N _lG) with the functional state of kidneys and biochemical composition of blood in I type membranous-proliferative glomerulonephritis (MPGN) in man. PATIENTS AND METHODS. The investigation was performed in 201 type MPGN patients. The content of cationic protein neutrophils was studied by the lysosomal-cationic test (LCT). Expression of erythrocyte, complement and Fcy-receptors on the neutrophil membrane was studied by the rosette-formation method. RESULTS. The value of the cytochemical LCT index positively correlates with the level of Ieukocyturia and negatively - with the glomerular filtration rate (GFR) and circadian proteinuria. Intensity of the E-receptor expression on NG membranes has a correlative association with the concentration of serum creatinine. C3b-receptor expression activity on the NG membrane is correlatively associated with the changes in GFR. The functional changes in NG correlate with GFR, intensity of Ieukocyturia and concentration of serum creatinine revealed 0.5-2 months after the investigation of NG. The changes of GFR, levels of Ieukocyturia and concentration of serum creatinine appeared to be delayed in time, since the changes of NG activity in peripheral blood were investigated ratherthan renal tissue infiltrate where the pathological reaction developed. CONCLUSION. The data presented allow a suggestion of the participation of NG in the mechanisms of lesions of the renai tissue in patients with I type MPGN. The revealed combined changes of activity of E-receptor expression on NG and the values of the cytochemical LCT index can be considered a prognostic sign in this disease warning about the following aggravation of renal functions.

1. Cameron JS, Turner DR, Heaton J et al. Idiopathic mesangiocapillary glomerulonephritis. Comparison of types I and Il in children and adults and long-term prognosis. Am J Med 1983; 74: 175-192

2. Peters DK, Martin A, Weinstein A et al. Complement studies in membranoproliferative glomerulonephritis. Clin Exp Immunol 1972; 11: 311-320

3. Lefkowith JB. Leukocyte migration in immune complex glomerulonephritis: role of adhesion receptors. Kidney Inter 1997; 51(5): 1469-1475

4. Huang XR, Holdsworth S.R., Tipping PG. Th2 responses induce humorally mediated injury in experimental anti-glomerular basement membrane glomerulonephritis. J Am Soc Nephrol 1997; 8 (7):1101-1108

5. Sheerin NS, Springalf Carrof MC et a(. Protection against anti-glomerular basement membrane (GBM) -mediated nephritis in C3-and C4-deficient mice. Clin Exp Immunol 1997; 3:403-409

6. Fujigaki Y, Nagase M, Kojima K et al. Glomerular handling of immune complex in the acute phase of active in situ immune complex glomerulonephritis employing cationized ferritin in rats. Ultrastructural localization of immune complex, complements and inflammatory cells. Virchow’s Archiv 1997; 431(1); 53-61

7. Matzner Y., Bar N.M., Yahalom J et al. Degradation of heparan sulfate in the subendothelial extracellular matrix by a readily released heparanase from human neutrophils: Possible role in invasion through basement membranes. JCiin Invest 1985;76:1306-1313

8. Janoff A. Neutrophil proteases in inflammation. Ann Rew Medicine 1972; 23:177-190

9. Janoff A. Elastase in tissue injury. Ann Rew Medicine 1985;36:207-216

10. Travis J, Savesen GS. Human plasma proteinase inhibitors. Ann Rev Biochem 1983; 52: 655 -709

11. McIntyre TM, Zimmerman GA, Prescott SM. Leukotrienes C4 and D4 stimulate human endothelial cells to synthesize platelet-activating factor and bing neutrophils. Proc NatIAcad Sci USA 1986; 83: 2204 -2208

12. Yared A, Albrightson WC, Griswold D et al. Functional significance of Ieukotriene B4 in normal and glomerulonephritic kidneys. J Am Soc Nephrol 1991; 2: 45-56

13. Тареева HE. Механизмы прогрессирования гломе-рулонефрита. Tep арх 1996; 6: 5-11

14. Пигаревский BE. Лизосомально-катионный тест. В: Пигаревский BE, ред. Клиническая морфология нейтрофильных гранулоцитов. Л.; 1988: 87-102

15. Петрова ИВ, Шкурко ВИ, Арапова ОЮ, Васильева Л П. Определение розеткообразующих клеток, комплемента и комплементфиксирующих антител в клинике аутоиммунных и иммунодефицитных заболеваний. Методические рекомендации. НИИ травматологии и искусственных органов М3 СССР. М., 1980

16. Новиков ДН. Справочник по клинической иммунологии и аллергологии. Беларусь Минск; 1987

17. Harlan JM. Neutrophil-mediated vascular injury. Acta Med Seand Suppl 1987; 751: 123-129

18. Lehrer RL, Lichtenstein AK, Ganz T. Defensins: Antimicrobial and cytotoxic peptides of mammalian cells. Annu Revlmmunol 1993; 11:105-128

19. Soma J, Saito T, Ootaka T et al. Differences in glomerular leukocyte infiltration between IgA nephropathy and membranoproliferative glomerulonephritis. Nephrol Dial Transplant 1998; 13: 608-616

20. Bainton DF. Developmental biology of neutrophils and eosinophils. In: Gallin Jl, Snyderman R, ed. Inflammation: basic principles and clinical correlates, 3 rd. Lippincott Williams & Wilkins, Philadelphia: 1999; 13-34