REVERSIBILITY OF STRUCTURAL CHANGES OF THE RATS KIDNEY MEDULLA CAUSED BY SUBCHRONIC INTAKE OF ETHYLENE GLYCOL

Abstract

THE AIM of the investigation was a complex morpho-functional study of the action of ethylene glycol on the kidney medullary substance, estimation of reversibility of the resulting changes and specificities of the distribution of calcium deposits under conditions of experimental nephrolithiasis. MATERIAL AND METHODS. The experiment was carried out in 20 male Wistar rats, body mass 200-250 g. The animals were divided into 4 groups, 5 rats in each. Rats of the 1st group were given pure water, 2nd group  -  1%  of ethylene glycol during 21 days that induced the development of experimental nephrolithiasis. The 3rd group animals, previously given ethylene glycol during 21 days, after this period received 2% starch suspension with a preparation from tissue culture of Rubia Cordifolia in dosage 75 mg/kg during 3 weeks. The 4th group animals simultaneously received the preparation from Rubia Cordifolia and a 1% solution of ethylene glycol during 21 days. RESULTS. The work has shown reversibility of the morphological alterations in the kidney caused by subchronic effects of ethylene glycol, and protective action of the preparation from tissue culture of Rubia Cordifolia. CONCLUSION. Administration of the preparation from tissue culture of Rubia Cordifolia results in reversible morphological alterations to the medullary substance of the kidney caused by subchronic intake of ethylene glycol.