ON THE ROLE OF PROCESSES OF FREE RADICAL OXIDATION IN THE DEVELOPMENT OF EXPERIMENTAL NEPHROLITHIASIS
https://doi.org/10.24884/1561-6274-2008-12-1-58-63
Abstract
THE AIM of the investigation was to study the process of free-radical oxidation in the kidneys and blood of rats with experimental urolithiasis. MATERIAL AND METHODS. Experiments were carried out in male Wistar rats given 1% solution of ethylene glycol in drinking water during 21 days. The presence of calcium oxalate crystals in kidney slices was determined histochemically Biochemical methods were used to assess the oxidant (thiobarbiturate reactive products, total oxidant activity) and antioxidant (catalase, superoxiddismutase, glutathione peroxidase, total antioxidant activity) status of the kidneys and blood. RESULTS. Considerable number of Ca-positive crystals localized mainly on the surface of the renal papilla was determined in kidney slices of all rats receiving ethylene glycol. Progression of nephrolithiasis was accompanied by intense activation of free-radical oxidation both in the kidneys and in blood. Abatement of antioxidant defense was simultaneously observed due to the inhibition of activity of glutathione peroxidase. CONCLUSION. Thus, activation of free-radical oxidation in the kidneys and blood of animals takes place under conditions of experimental oxalate nephrolithiasis in rats. This process is followed by weakened activity of glutathione peroxidase as one of the main antioxidant enzymes.
About the Authors
Ya. F. Zverev
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
V. M. Bryukhanov
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
O. S. Talalaeva
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
V. V. Lampatov
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
A. Yu. Zharikov
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
S. V. Talalaev
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
Ya. S. Bulgakova
Алтайский государственный медицинский университет
Russian Federation
Russian Federation
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Review
For citations:
Zverev Ya.F., Bryukhanov V.M., Talalaeva O.S., Lampatov V.V., Zharikov A.Yu., Talalaev S.V., Bulgakova Ya.S. ON THE ROLE OF PROCESSES OF FREE RADICAL OXIDATION IN THE DEVELOPMENT OF EXPERIMENTAL NEPHROLITHIASIS. Nephrology (Saint-Petersburg). 2008;12(1):58-63. (In Russ.) https://doi.org/10.24884/1561-6274-2008-12-1-58-63
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