EXPERIMENTAL MODEL OF INTERSTITIAL RENAL FIBROSIS

A. V. Smirnov; G. T. Ivanova; O. N. Beresneva; M. M. Parastaeva; V. G. Sipovskiy; M. I. Zarayskiy; I. J. Saburova; E. B. Sipovskaya; I. G. Kayukov

doi:10.24884/1561-6274-2009-13-4-70-74

EXPERIMENTAL MODEL OF INTERSTITIAL RENAL FIBROSIS

A. V. Smirnov, G. T. Ivanova, O. N. Beresneva, M. M. Parastaeva, V. G. Sipovskiy, M. I. Zarayskiy, I. J. Saburova, E. B. Sipovskaya, I. G. Kayukov

https://doi.org/10.24884/1561-6274-2009-13-4-70-74

Full Text:

PDF (Rus)

Generate QR code

Abstract

THE AIM. Assess the impact of unilateral ureteral obstruction (UUO) in Wistar rats. MATERIAL AND METHODS. Unilateral ureteral ligation left kidney performed in 6 Wistar rats. Time of observation after the UUO was 14 days. Experimental animals to measure the mass of the kidneys and the level of mean arterial pressure (BP), held light microscopic morphological study and determined the level of expression of the nuclear transcription factor (NFкB) in renal tissue. RESULTS. No significant change in the mean arterial pressure in rats 2 weeks after surgery compared with preoperative levels. Mass left kidney increased after the operation almost 4.5 times for the control right kidney. The relative level of expression of the nuclear transcription factor NFкB in experimental left kidney in 1.5 times higher than in the contralateral organs. It is noted thinning of the cortical and medullary layers, dilatation of the lumen of proximal tubules with flattening and desquamation of the epithelium, degeneration of the epithelium of proximal tubules, the accumulation of lymphocytes-macrophages with the growth of connective tissue in extracellular matrix and stromal edema. In glomeruli involved, area-defined expansion of the urinary space of Bowman capsule, compression and partial collaps of the capillary loops. CONCLUSION. These data suggest that the unilateral ureteral ligation in rats after 14 days leads to a distinct damage of kidney tissue, which are mainly localized in the tubulointerstitial compartment.