INFLUENCE DICHLORIDE OF MERCURY ON OSMOTIC RESISTANCE OF ERYTHROCYTES

Abstract

THE AIM. The purpose of this study was to examine the influence of different concentrations of mercury dichloride on osmotic resistance of human erythrocytes in vitro investigations and possible mechanisms of hemolysis of cells. MATERIALS AND METHODS. Determination of osmotic resistance of erythrocytes was carried out on healthy people for a unified method to L.I. Idelson modification. RESULTS. Installed a dose-dependent nature of HgCl₂ on the osmotic resistance of human erythrocytes: small concentrations (0.05-0.1 mmol/L) have a moderate hemolytic, medium (0.2-1.0 mmol/L) - blocking, and large (> 1.0 mmol/L) – a destructive effect on the cells. Statistically significant (p <0.001) reduction of the intensity of hemolysis of erythrocytes in hypotonic medium with mercury, most likely due to the blockade of aquaporins. CONCLUSION. Osmotic resistance of red blood cells is reliable and reproducible index of functional state of the cell, sensitive to the chemical effect. as shown by the example of exposure of erythrocytes HgCl₂ in a wide range of concentrations, differing by three orders of magnitude. In further studies to establish the time parameters of the observed effects of mercury on the osmotic fragility test, the likely participation and contribution of other biochemical mechanisms, as well as to compare the data with the osmotic resistance of various types of cells to mercury and other heavy metals, aquaporin blockers.