FUROSEMIDE DIURETIC ACTIVITY IN RATS ON THE BACKGROUND OF MEDICINAL DRINKING MINERAL WATERS WITH DIFFERENT ACIDITY INTAKE

Abstract

THE AIM. To evaluate the influence of mineral water Essentuki №17 and Essentuki №20 on the diuretic activity of furosemide in rats. MATERIALS AND METHODS. Studies conducted on male rats Wistar, which were maintanied on a standard diet. For two weeks, the animals in group I received as drinking tap water, II group – alkaline mineral water Essentuki №17, III group – slightly acid mineral water Essentuki №20. Once furosemide was administered subcutaneously at a dose of 10 mg / kg, for a duration of 5 days to 5 mg / kg. The measurement of pH of urine, daily diuresis, urinary excretion of sodium and potassium was perfprmed. RESULTS. These pH-metry before the experiment in all three groups showed the value of 7,2 ± 0,2. After two-week drinking water treatment in groups I and III, significant changes in pH of urine were found. In Group II with mineral water Essentuki №17, urine pH increased to 8.0. Difference in magnitude of the diuretic effect of furosemide in animals of all three groups was not identified. CONCLUSION. These results suggest that diuretic and saluretic activity of furosemide does not change during treatment with therapeutic mineral waters Essentuki №17 and Essentuki №20. Apparently, they cause changes of acid-base composition of the urine of rats are not sufficient for significant changing balance between ionic and molecular forms of furosemide, and therefore – for the corresponding changes in activity of the diuretic.