EFFECT OF THERMAL ISCHEMIA-REPERFUSION ON EXPRESSION OF APOPTOSIS-REGULATING GENES IN THE RENAL TISSUE OF PATIENTS WITH RENAL CELL CARCINOMA

Improved knowledge of molecular mechanisms of the damaging effect of thermal ischemia and reperfusion to renal tissue of patients with renal cancer has significant prospects for new therapeutic approaches aimed at enhancing quality of care. THE AIM: to study changes in the expression of apoptosis-regulating genes MDM 2, BAX, CASP7, CASP3, CASP8, CASP9, BCL2, CASP8 / FADD, p53, APAF1, AIFM1, ICAD and XIAP in renal tissue of patients with renal cell carcinoma subjected to the action of ischemia and reperfusion. PATIENTS AND METHODS. We used for the study tissue biopsies of 12 patients with histologically confirmed diagnosis of renal cancer. Needle biopsy was performed before stop the blood supply, for 10 minutes of ischemia and 20 minutes after reperfusion in the kidney. The relative expression of genetic loci was determined by real-time PCR. RESULTS. It was found: 1) absence from the 10th minute of ischemia significant differences transcriptome profile of the majority of investigated genes from similar parameters prior to ischemia, with the exception of reducing expression of genes CASP7 and ICAD; 2) a significant increase in expression of pro-apoptotic genes (BAX, CASP3/7, APAF1 and p53), and anti-apoptotic genes (XIAP, MDM2 and BCL2) 20 minutes after reperfusion of the kidney tissue; 3) a changes in the balance of expression of pairs of proand anti-apoptotic genes p53 / MDM2 and Bax / BCL2 in the 10th minute of ischemia and 20 minutes after reperfusion. CONCLUSION. These data characterize transcriptomic state of renal tissue in the early period after ischemia and restore the blood flow in it as the initiation point shift the balance of proand anti-apoptotic genes. 

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