DOSE-DEPENDENDENT HISTOCHROME EFFECTS OF KIDNEY IN RATS

Abstract

THE AIM. Histochrome is a native antioxidant drug isolated from natural sourse. The study of pharmacological activity of histochrome showed a wide spectrum of dose-range action. The aim of this study was to investigate the renal effects changes when administered various doses of histochrome in rats. MATERIALS AND METHODS. The study was conducted on outbred stock Wistar rats. The test group of animals (n = 15) was administered subcutaneously histochrome at a dose of 1 mg/kg for 10 days, and the control group (n = 20) was treatment of 10 mg/kg of the drug. Since 3-d day every two days of experiment were measured daily urine output, excretion of Na+ and K+, creatinine excretion, and excretion of histochrome. RESULTS. The tendency of daily urination increase recorded at histochrome administration at a dose of 1 mg/kg. In the comparison group increased diuresis led to a fivefold magnification of parameter on the 7th day. Dynamic renal creatinine excretion during treatment with 1 mg / kg histochrome had a stable character throughout the experiment, while the ten-fold increase in dose was associated with a significant elevation of the factor. Natriuresis steadily increased, exceeding the initial value 5 times in under-test group of animals. In the comparison group the ion excretion increased by 2 times. Potassium excretion have similar dynamics using both histochrome doses. The native form histochrome was not detected in the urine in any of the animal groups. CONCLUSION. The experimental results showed that the behavior of the excretory renal function is histochrome dosedependent and may be due to its metabolites.