THE ROLE OF GLUCOCORTICOID DYSREGULATION IN THE DEVELOPMENT OF GESTATIONAL PYELONEPHRITIS, PLACENTAL INSUFFICIENCY IN THE EXPERIMENT

Abstract

THE AIM of the study was to examine the mechanisms of development of gestational pyelonephritis and placental insufficiency in the conditions of the experiment.

MATERIAL AND METHODS. Biological modeling conducted on 40 pregnant female rats of Wistar Kyoto line weighing 250-300 gr. In the first block of the experiment [I group (n=10), group II (n=10), control] have made glucocorticoid dysregulation through the use of glucocorticoid drug “prednisolone” from 3rd to 11th day of gestation (by method of Yu.Yu.Chebotareva, V.G. Ovsyannikov et al.) followed by determination of morphological changes of the fetus (weight, length) and histological analysis of the kidneys and Feto-placental complex. In the second block of the experimental study [III (CC) group (n=10), group IV (n=10), control] on the same background with 1 unit of glucocorticoid drug studied the specific features of pregnancy, labor, the condition of newborn rats (height, weight, developmental abnormalities). Used in this experiment methods of anesthesia, the killing of rats corresponds to the principles of the Helsinki Declaration about the humane treatment of animals. The experiment was carried out, taking into account international standards and requirements for use in non-clinical studies quality animals in accordance with the Principles of good laboratory practice (national standard of Russian Federation GOST R 53434-2009, March 2010), which provides the conducting of all preclinical, extraclinical and expert researchers in accordance with international standards of GLP.

RESULTS. It is revealed that the experimental simulation of disorders of reproductive function in rats by hormonal balance violations forms gestational pyelonephritis and placental insufficiency, which is manifested by the increased number of aborts, intrauterine growth retardation of fetuses, decrease their weight.

CONCLUSION. In the present experimental study refined the role of glucocorticoid dysregulation in the development of gestational pyelonephritis, placental insufficiency.