DETERMINATION OF URINARY EXCRETION OF MONOCYTE HAEMOATTRACTIVE PROTEIN-1 IN RENAL DISEASES IN CHILDREN

Abstract

THE AIM OF THE STUDY - to investigate the features of urinary excretion of MCP-1 in children with various kidney diseases PATIENTS AND METHODS. The study included 25 healthy children, 22 patients with nephrotic syndrome, 19 - with chronic glomerulonephritis with hematuria, 6 - with nephritic syndrome of acute glomerulonephritis, 9 - with acute renal failure, and 2 -with chronic renal failure. Along with traditional methods it was conducted immunological examination including determination of the concentration of serum antibodies (A, F, G,); levels of circulating immune complexes, complement, lymphocyte immunophenotyping. According to the DNA-cytometry of peripheral blood there were assessed ploidy and cell cycle kinetics. The level of MSR-1 in urine was determined by enzyme immunoassay using mono-and polyclonal antibodies (ZAO Vector-Best Novosibirsk). RESULTS. It was established that in the acute period of all forms of renal pathology MCP-1 rise in the urine. As a result of correlation analysis it was revealed association opened the etiopathogenetic mechanisms of disease progression. It was identified criteria to monitor the activity of the process, determine prognosis, treatment strategy and evaluate the effectiveness of therapy. CONCLUSION. Results of the study suggest that detecting the level of urinary excretion of MCP-1 may be used for monitoring process activity and for determining treatment strategy and evaluating the effectiveness of therapy in glomerulonephritis in children.