PATTERNS OF THE «DOSE-EFFECT» RELATIONSHIP FOR FUROSEMIDE AT RATS

Abstract

THE AIM. To detect possible relationship of furosemide pharmacological effect on kidneys function at rats from different diuretic doses with "dose-effect" response graphing. MATERIAL AND METHODS. The research was done at male Wistar rats. Daily factors of urinary passage and electrolyte excretion were determined after single subcutaneous injection of furosemide with doses 0,5, 1, 2, 5, 10, 20 mg/kg. RESULTS. It provides suggestions that furosemide complexing with NKCC happens in the range of 1-2 mg/kg doses. Saturation of potassium excretion transfers was registered in the range from 2 to 5 mg/kg on retention of sodium excretion increment linearity. In a range from 5 to 20 mg/kg sequential sodium and chlorine excretion increase was observed at invariable potassium excretion level. CONCLUSION. "Dose-effect" dependence received data analysis allowed to reveal a number of furosemide diuretic and saluretic activity laws. Sodium ions excretion reaches first saturation threshold at furosemide doses from 0,5 to 2 mg/kg, then at high doses further slow allocation increase of these ions is observed. Potassium ions excretion dependence of furosemide dose differs from sodium excretion. The curve has one saturation threshold, which is reached at doses from 1,5 to 5,0 mg/kg, and at higher doses potassium ions excretion is invariable. Chloride ions excretion is proportional to total potassium and sodium ions excretion tellingly to all cation-chlorine transporters series which are carrying out electroneutral transport