ГОМОЦИСТЕИН ВЫЗЫВАЕТ ПОВРЕЖДЕНИЯ НЕ ТОЛЬКО КЛУБОЧКОВОГО, НО И КАНАЛЬЦЕВОГО ОТДЕЛА НЕФРОНА (ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ)

ЦЕЛЬЮ ИССЛЕДОВАНИЯ было выявление функциональных и структурных последствий экспериментальной гипергомо цистеинемии в почках крыс. МАТЕРИАЛ И МЕТОДЫ. Взрослым белым крысам линии Вистар в течение 3 недель паренте рально вводили гомоцистеин (ГЦ; 13.4 мг/кг) для оценки влияния экспериментальной гипергомоцистеинемии (ГГЦ) на мочевую экскрецию альбумина (МЭА), клиренс креатинина (CCr), а также на структуры клубочков и проксимальных каналь цев (ПК) на светооптическом и электронномикроскопическим уровнях. РЕЗУЛЬТАТЫ. Развитие умеренной ГГЦ у экспери ментальных животных приводило к достоверному увеличению МЭА без достоверного изменения CCr, что сопровождалось умеренной мезангиальной пролиферацией, гиперплазией гломерулярной базальной мембраны, заметным увеличением числа больших апикальных вакуолей и вторичных лизосом, а также очаговой утратой щеточной каймы в отдельных клетках ПК. Кроме того, в ПК было отмечено увеличение количества аутофагосом, наряду с наличием эпителиальных клеток с дезорганизованными участками базальной цитоплазмы. ЗАКЛЮЧЕНИЕ. Полученные данные следует рассматривать как прямое доказательство повреждающего действия ГГЦ на клубочки и клетки ПК.

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