MORPHOLOGICAL AND HISTOCHEMICAL ALTERATIONS IN THE KIDNEY TUBULE EPITHELIUM IN MODELING ACUTE METHANOL POISONING

Abstract

THE AIM of the investigation was to study morphohistochemical alterations of the kidney tubule epithelium of experimental animals after acute poisoning with methyl alcohol. MATERIAL AND METHODS. A lethal dose of methanol was introduced through a probe into the stomach of 30 white rats of both sexes. Renal tissue sections were stained with hematoxylineosin and picrofuchsin after VanGieson. Activity of succinate dehydrogenase, lactate dehydrogenase, NADH 2dehydrogenase, acid phosphatase and alkaline phosphatase was detected on sections frozen in liquid nitrogen. RESULTS. Introduction of methanol was found to result not only in severe structural alterations of the organ, but also in a complex impairment of intracellular metabolism of the proximal and distal tubule nephrothelium and functional state of the microcellular bed which can be taken for early signs of toxic effects of methanol and development of acute renal failure. CONCLUSION. Methanol has a direct nephrotoxic effect resulting in structural metabolic impairments which can be a basis for the development of nephropathy with symptoms of acute renal failure.