Study of protective heat shock proteins in urine, serum and kidney tissue in patients with chronic glomerulonephritis
https://doi.org/10.24884/1561-6274-2015-19-2-92-108
Abstract
THE AIM is to clarify the value of determination of heat shock proteins (HSP) in urine to assess the activity and prognosis of CGN. PATIENTS AND METHODS. Study included 75 patients with active forms of chronic GN: group 1 -34 patients with bladder syndrome and group 2 - 41 patient with nephrotic syndrome who have urinary syndrome was the result of incomplete remission of nephrotic syndrome. The comparison group of 10 patients with proteinuria < 0.5 g/day and 10 healthy. Method ELISA) in serum was determined by the level of antibodies to HSP-70 in urinary levels of HSP-27 and HSP-70, IL-6 and IL-10. In kidney tissue (peroxidase immunohistochemical method) was determined by the expression of HSP-70 in various structures; the expression of FoxP3. RESULTS. Excretion of HSP-27 and -70 urine was increased by increasing the activity of CGN, Established direct correlation to the concentration in the urine HSP-27 and -70 with signs of activity CS. CONCLUSION. Confirmed kidney «self-defense» mechanisms capability assessment using the study of one of its parts - HSP.
About the Authors
N. V. Chebotareva
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
N. V. Neprintseva
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
I. N. Bobkova
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
L. V. Kozlovskaya
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
V. A. Varshavskii'
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Chebotareva N.V., Neprintseva N.V., Bobkova I.N., Kozlovskaya L.V., Varshavskii' V.A. Study of protective heat shock proteins in urine, serum and kidney tissue in patients with chronic glomerulonephritis. Nephrology (Saint-Petersburg). 2015;19(2):55-62. (In Russ.) https://doi.org/10.24884/1561-6274-2015-19-2-92-108
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