FUNCTIONAL MORPHOLOGY OF THE PROXIMAL AND DISTAL CHANNELS OF THE KIDNEYS IN MODELING ACUTE LETHAL POISONING WITH GAS

Abstract

THE AIM of the work was to study histological and histochemical alterations to the kidneys in modeling poisonings with domestic gas in experiment. MATERIAL AND METHODS. Changes in activity of NAD-H2-dehydrogenase, lactate dehydrogenase (LDG), succinic dehydrogenase (SDG) and alkaline phosphatase (APh) were estimated in the proximal and distal channels of the kidneys of white rats in modeling poisoning with domestic gas. Activity of the enzymes was estimated by quantitative cytospectrophotometric method in perinuclear parts of nephrotelium cell cytoplasm. RESULTS. The histochemical investigation of the epithelium of convoluted channels of the kidneys of experimental animals allowed to detect substantial changes of metabolism of cells characterized by impaired energy metabolism of tissue ventilation and transportation processes. A histoenzymological analysis has shown that toxic exposure to the gas resulted in a reliable reduction of LDG activity as compared with the control. Activity of SDG was also changed, but was considerably higher as compared with LDG. Activity of NAD-H2-dehydrogenase was considerably increased as compared with activity of this enzyme in the group of control animals. Poisoning with the gas resulted in higher activity of APh as compared with activity of the enzyme registered in control rats. CONCLUSION. The process of intoxication with domestic gas was followed by morphofunctional alterations of the kidneys which can lead to the development of renal failure.