Epithelial-mesenchymal transition as an aspect of kidney reparation in context of chronic nephropathy

Abstract

AIM: to identify relationship between epithelial-mesenchymal transition (EMT) and renal parenchyma injury and renal dysfunction. PATIENTS AND METHODS. The study included 62 patients with various forms of chronic glomerulonephritis (GN), 25 males and 37 females. To assess phenotype of epithelial cells used monoclonal antibodies (company DAKO, ready-to-use (RTU) to pancytokeratine (clone AE1 / AE3), E-cadherin (clone NCH-38), CD10 (clone 56C6), as marker for mesenchymal differentiation used monoclonal antibodies to vimentin (clone V9) and alpha-smooth muscle actin (clone 1A4). Evaluation of tubular epithelium proliferative activity was performed using antibodies to Ki67 (clone MIB-1). RESULTS. According to the study EMT was observed in 66% patients, in 34% this phenomenon is not found. Tubulointerstitial fibrosis (TIF) had moderate severity in 84%, in 11% cases was significant, in 5% of patients not found. In patients group with IgA-nephropathy and FSGS, during logistic regression analysis without EMT risk of ESRF was 8%, with moderate EMT end-stage renal failure risk increased to 55% (df = 1 , p = 0,003). CONCLUSIONS. Thus, according to results of the study, in patients with EMT severe CKD stages are significantly more common.