KIDNEY FUNCTION IN EXPERIMENTAL DIABETES

Abstract

THE AIM. To study the renal function of rats in conditions of streptozotocin-induced model of experimental diabetes mellitus. MATERIAL AND METHODS. The study was conducted on 25 male Wistar stock rats (12 individuals – a group of intact rats and 13 individuals – a disease control group). Diabetes mellitus was simulated by intraperitoneal administration of Stretpozotocin at a dose of 65 mg / kg. For a more selective simulation of type II diabetes, rats of the disease control group were injected intraperitoneally with a cytoflavin solution at the rate of 115 mg / kg nicotinamide dosage. In both groups, the daily urine volume was collected every 7 days during the month, in which the concentrations of glucose, protein, and creatinine were determined. Taking into account the daily volume of diuresis, excretion of glucose, protein, and creatinine was calculated. RESULTS. At baseline, all determinable indicators of renal function in rats did not differ between groups. Then, in the disease control group, there was a significant increase in daily diuresis, protein excretion, glucose excretion, and creatinine. As a result, by the end of the experiment, the magnitude of these indicators exceeded the level of intact rats by 2.0; 1.5, 3.2 and 1.9 times, respectively, CONCLUSION. Under conditions of the streptozotocin model of diabetes mellitus, characteristic changes in the renal excretory function are observed: an increase in daily diuresis, an increase in the excretion of glucose, an excretion of protein and creatinine.