Does the urine microbiota of healthy women vary during daytime?

Abstract

THE AIM: to study the urine microbiota of healthy women during daytime. PATIENTS AND METHODS. We performed bacteriological examination of urine samples from 20 healthy sexually active women taken at different time (at 8:00, 12:00, and 16:00). Examination of the urine was performed using an extended set of culture media for facultative anaerobic and non-clostridial anaerobic bacteria. 10 culture media were used. HiAnaerobic System - Mark VI or AnaeroHiGas Pak were used to create anaerobic conditions. Calculations were performed in R software environment for statistical computing and graphics. RESULTS. Bacteriological examination of 60 urine specimens revealed no sterile samples. In the facultative anaerobic bacteria group, coagulase-negative staphylococci and Corynebacterium spp. were detected with high frequency (>60,0%) throughout the day. In the nonclostridial anaerobic bacteria group, Eubacterium spp., Peptococcus spp., Propionibacterium spp., and Lactobacillus spp. demonstrated stable association. Bacteriuria level for all microorganisms verified in the urine of healthy women in the overwhelming majority of cases was <10³ CFU/ml. Cochran’s test established the absence of significant variations in the detection frequency of different bacterial genera during the day. Except for 3 genera, no significant differences in the bacteriuria levels were revealed. Dendrograms indicate high level of similarity between the presence frequency and concentration of microorganisms at different points in time. CONCLUSION. Typical spectrum of the urine is identified in healthy women irrespectively of the time of the day.