The mechanisms of ion regulation kidneys function in histohemic hypoxia and possible ways of its correction

Abstract

THE AIM OF RESEARCH was to study the mechanisms of ion regulation in the kidneys under the conditions of histohemic hypoxia (HHH), as well as the nephroprotective properties of diltiazem and flocalin. MATERIALS AND METHODS. The experiments were made on non-linear laboratory whitw rats with 0.15-0.17 kg weight after 5% water load. HHH was modeled by administration of sodium nitrite at a dose of 50 mg/kg and 2,4-dinitrophenol at a dose of 3 mg/kg. After 30 days we administered diltiazem and flocalin intragastrically at a dose of 5 mg/kg each on 1% starch solution during 7 days. Concentration of sodium and potassium ions in urine and blood plasma was measured with flame photometry, and creatinine concentration was measured with colorimetry technique. RESULTS. Progression of histohemic nephropathy in rats was characterized by the decrease of glomerular filtration rate (GFR), decrease of filtrational sodium load, inhibition of tubular sodium reabsorption, increase of natriuresis and kaliuresis. After administration of diltiazem the studied indexes of renal function in rats with HHH did not change. Conversely under the influence of flocalin GFR and filtrational sodium load increased, water reabsorption restored, proximal transport of sodium ions increased, kaliuresis decreased. CONCLUSION. Assessment of the renal effects of the representatives of calcium and potassium channels has shown that unlike diltiazem, flocalin can be regarded as a nephroprotective agent under the conditions of histohemic hypoxic lesion of kidneys.