THE RENOTROPIC EFFECTS OF ADENOSINE TRIPHOSPHATE-SENSITIVE POTASSIUM CHANNEL ACTIVATION DEPENDING ON THE FUNCTIONAL STATE OF KIDNEYS IN RATS

Abstract

THE AIM of the study was to: research of renotropic effects of adenosine triphosphate-sensitive potassium channels activator Flocalin. MATERIAL AND METHODS. The experiments were made in laboratory non-linear white rats 0,15-0,17 kg of weight under conditions of 5% water load. The first series of experiments consisted of intraventricular administration of Flocalin with a tube in the dose of 5 mg/kg on 1% amylum mucus over the period of 7 days. In the second series of experiments Flocalin was administered once in 2 hours after modeling of toxic nephropathy by subcutaneous injection of mercuric chloride 1 mg/kg. Urine and plasma levels of sodium and potassium ions were measured using flame photometry. Quantitative analysis of creatinine was performed with colorimetric assay. Urine protein was estimated using sulfosalicylic acid test. RESULTS. The repeated administration of Flocalin to the rats increased diuresis, as well as creatinine excretion and glomerular filtration rate (GFR). We observed elevation of plasma potassium levels and diminution of urine potassium concentration. At the same time plasma sodium levels and natriuresis decreased. After Flocalin administration in the model of sublimate nephropathy we observed the increase of diuresis, creatinine excretion and GFR, decrease of proteinuria and elevation of urine potassium levels and kaliuresis. This was followed by plasma sodium level rate normalization and increment of potassium ions excretion in the urine. CONCLUSION. A more pronounced reaction of water excretion after administration of Flocalin to the rats under conditions of sublimate nephropathy contributes to the increase of renal sodium and potassium excretion with the absence of electrolyte changes in blood plasma. Elevation of GFR and antiproteinuric effect determine renoprotective action of Flocalin in toxic nephropathy.