THE STATE OF THE GLUTATHIONE SYSTEM IN PARENCHYMAL ORGAN TISSUES OF LABORATORY ANIMALS AFTER REPEATED ADMINISTRATION OF CYCLOPHOSPHAMIDE

THE AIM of the investigation was to carry on a complex study of the state of the glutathione system and intensity of lipid peroxidation processes in the liver and kidney tissues in white rats after repeated administration of cyclophosphamide (daily during 3-10 days, in doses 20 and 40 mg/kg in each injection). MATERIAL AND METHODS. The concentration of reduced glutathione, sulfhydryl groups of proteins, malondealdehyde, diene conjugates and activity of glucose-6-phosphate dehydrogenase, glutathionereductase, glutathioneperoxidase, glutathione-S-transferase, catalase was determined spectrophotometrically in tissues of 50 animals. RESULTS. It was shown that this form of intoxication was accompanied by pronounced changes in the state of the glutathione system in the liver and kidney tissue of the poisoned animals (lower content of sulfhydryl groups of proteins, impaired activity of glutathionereductase, glucose-6-phosphate dehydrogenase, glutathione-S-transferase, glutathioneperoxidase), activation of lipid peroxidation processes. The causes of the appearance of these biochemical shifts, their tissue distinctions were discussed as well as their role in realization of the cytotoxic effect of cyclophosphamide. CONCLUSION. The pathogenetic role of a depletion of functional possibilities of the glutathione system was established as well as activation of free radical processes in realization of the cytotoxic effect of alkylating drugs.

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