CELLULAR COMPOSITION OF BLOOD AND BONE MARROW OF RATS AFTER ADMINISTRATION OF CYCLOPHOSPHAMIDE AND IRRADIATION OF SKIN WITH EXTREMELY HIGH FREQUENCY WAVES

THE AIM of the research was to monitor the content of blood cells and bone marrow of rats in 24 hours and 7 days after a single intraperitoneal injection of cyclophosphamide (20 and 40 mg of body weight of the animal) and to attempt correction of the observed alterations using irradiation of the skin with extremely high frequency (EHF) waves. MATERIAL AND METHODS. The experiments were performed in 50 male Wister rats. The quantity of blood leukocytes and bone marrow myelocytes were determined in 24 hours and 7 days after injection of cyclophosphamide (20 and 40 mg), EHF irradiation of the skin (37-53 GHz, 7.1 mm, 20 mWt), and a combination of these factors. RESULTS. A single i.p. injection of cyclophosphamide was shown to result in a decreased quantity of blood neutrophils and lymphocytes, lymphoid cells and total quantity of nondifferentiated cell elements of the bone marrow in 24 hours after injection. Lymphopenia and increased number of nondifferentiated cell elements of the bone marrow were noted in 7 days after injection. EHF irradiation of skin leads to the increased quantity of band neutrophils in blood and decreased band and segmental neutrophils in bone marrow of rats in 24 hours and levels the cyclophosphamide-induced increase of the quantity of nondifferentiated cells in bone marrow in 7 days. CONCLUSION. Injection of cyclophosphamide causes cytopenia in blood and bone marrow of rats in 24 hours. In 7 days after injection of cyclophosphamide the quantity of mature cells remains diminished, but the number of nondifferentiated cell forms is increased. EHF irradiation of skin leads to decreased intensity of the observed alterations.

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