POSSIBILITIES OF MOLECULAR-GENETIC RESEARCH FOR PREDICTION OF BLADDER CANCER
https://doi.org/10.24884/1561-6274-2010-14-2-67-71
Abstract
THE AIM – to examine FGFR3 gene mutation and expression of p53 gene to determine the prognosis of patients with bladder cancer. PATIENTS AND METHODS. A total of 25 patients with bladder cancer were investigated. Materials for molecular genetic studies were surgical specimens. Genomic DNA from tumor and blood were obtained using a set GenElute (USA), and mutational damage was determined in the genes FGFR3 (exon 7) and p53 (exons 5, 6, 7). RESULTS. According to our data, mutations of exon 7gena FGFR3 characterizes the favorable course of disease, and mutations in exons 5, 6, 7 p53 gene is observed in the poor course of bladder cancer. CONCLUSION. To determine the prognosis of bladder cancer is useful to determine the FGFR3 gene mutations and p53.
About the Authors
A. S. Al-Shukri
Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова
Russian Federation
Russian Federation
V. N. Tkachuk
Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова
Russian Federation
Russian Federation
M. V. Dubina
Санкт-Петербургский государственный медицинский университет им. акад. И.П. Павлова
Russian Federation
Russian Federation
References
1. Аль-Шукри СХ, Ткачук ВН. Опухоли мочеполовых органов: Руководство для врачей. «Питер», СПб., 2000; 320
2. Jemal A, Siegel R, Ward E et al. Cancer statistics, 2007. Cancer Clin 2007; 57:43-66
3. Аполихин ОИ, Какорина ЕП, Сивков АВ и др. Cостояние урологической заболеваемости в Российской Федерации по данным официальной статистики. Урология 2008; (3): 3-9
4. Павлов ВН, Казихинуров АА, Крупин ВН и др. Изменение ультраструктуры и микроциркуляции стенки мочевого пузыря у пациентов с неинвазивными формами рака. Онкоурология 2008; (4): 57-60
5. Kaufman D. Challenges in the treatment of bladder cancer. Ann Oncol 2006; 17: 106-112
6. Knowels M. Molecular subtypes of bladder cancer. Carcinogenesis 2006; 27(3): 361-373
7. Аль-Шукри АС, Ткачук ВН, Дубина МВ. Прогностические молекулярно-генетические маркеры рака мочевого пузыря. Онкоурология 2009; 2: 78-82
8. Bakkar A, Wallerand H, Radvanyi F et al. FGFR3 and p53 characterize alternative genetic pathways in the pathogenesis of urotelial cell carcinoma. Cancer Res 2003; 63(8): 8108-8112
9. Hernandez S, Lopez-Knowels E, Lloreta J et al. FGFR3 and Tp53 mutation in T1G3 transitional bladder carcinomas: independent distribution and lack of association with prognosis. Clin Cancer Res 2005; 11: 5444-5450
10. Green PM, Giannelli F. direct sequencing of PCR-amplified DNA. Molecular Biotechnology 1994; 2: 117-124
Review
For citations:
Al-Shukri A.S., Tkachuk V.N., Dubina M.V. POSSIBILITIES OF MOLECULAR-GENETIC RESEARCH FOR PREDICTION OF BLADDER CANCER. Nephrology (Saint-Petersburg). 2010;14(2):67-71. (In Russ.) https://doi.org/10.24884/1561-6274-2010-14-2-67-71
Views:
318
Email this article 