EXPERIENCE WITH USING CAPILLARY ELECTROPHORESIS FOR DETERMINATION OF THE CONCENTRATION OF PHOSPHATES IN THE USED DIALYZING SOLUTION

Abstract

THE AIM of the work was to assess the potential of using the method of capillary electrophoresis for determining the concentration of phosphates in the used dialyzing solution in the process of a hemodialysis (HD) procedure as an index of Ca-P metabolism. PATIENTS AND METHODS. A group of 7 patients (4 men and 3women, mean age 54.5±3.5 years) was examined. They received substitution renal therapy with regular hemodialysis for chronic renal failure (CRF). Every patient received on average 930±240 sessions of HD at the moment of examination. The patients were divided into two groups according to the content of parathyroid hormone (PTH): I group (3 patients) with low content of PTH, II group (4 patients) with high content of PTH. Phosphates were determined by the method of capillary electrophoresis with the device “Kapel-103 P” granted by company “Lumeks”. RESULTS. The level of excreted phosphate was maintained practically constant during the whole session. The correlation analysis has shown that of greatest significance are the interrelations of summary indices of elimination of phosphate with the concentrations of blood plasma protein components, as well as with activity of alkali phosphatase that characterizes the degree of bone tissue resorption. CONCLUSION. The obtained data of the phosphate elimination rate are of great clinical value. However the application of the method of capillary electrophoresis in this experiment seems to be too laborious and uneconomic for using in mass examinations.